PYOCYANIN PREPARATION FROM PSEUDOMONAS-AERUGINOSA ISOLATED FROM HETEROGENEOUS CLINICAL MATERIALS
PYOCYANIN PREPARATION FROM PSEUDOMONAS-AERUGINOSA ISOLATED FROM HETEROGENEOUS CLINICAL MATERIALS
复制标题
DOI：
10.1007/bf02928003
复制
发表时间：
1986-01-01
影响因子：
2.6
通讯作者：
TAHER, R
中科院分区：
文献类型：
--
作者：
ALANI, FY;ALSHIBIB, AS;TAHER, R
从不同临床标本分离的120株铜绿假单胞菌中制备了纯绿青素。改进后的方法使纯化率提高了425倍。所有菌株的pyocyanin光谱均一致，在363 nm处有一个主峰。这些光谱之间的唯一区别似乎是吸光度的水平值。从不同的临床标本中恢复的菌株在色素生产能力方面存在差异。从尿液中分离出的菌株比其他菌株产生更多的pyocyanin。
Pure pyocyanin was prepared from 120 strains of Pseudomonas aeruginosa isolated from different clinical specimens. The modified method used increased the purification 425-fold. The spectra of pyocyanin of all strains were found to be identical with a single major peak at 363 nm. The only difference between these spectra appeared to be the level value absorbance. Strains recovered from different clinical specimens differ in their ability for pigment production. The strains isolated from urine produced higher amounts of pyocyanin than other isolates.