A single starfish Aurora kinase performs the combined functions of Aurora-A and Aurora-B in human cells
A single starfish Aurora kinase performs the combined functions of Aurora-A and Aurora-B in human cells
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DOI：
10.1242/jcs.076315
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发表时间：
2010-11-15
影响因子：
4
通讯作者：
Kishimoto, Takeo
中科院分区：
文献类型：
--
作者：
Abe, Yusuke;Okumura, Eiichi;Kishimoto, Takeo
极光是一种重要的有丝分裂激酶，在进化过程中高度保守。大多数脊椎动物至少有两种极光蛋白，Aurora-A和Aurora-B，它们分别在中心体纺锤体和内部着丝粒中体中具有不同的功能。然而，一些非脊椎动物后口动物只有一个极光。单个极光是否与脊椎动物Auroras A和B的组合具有相同的功能还有待验证。我们已经从棘皮动物海星Asterina putinifera中分离到一个单一极光(Aurora，ApAurora)的cDNA，并表明ApAurora在海星卵母细胞和早期胚胎中显示了Aurora-A和Aurora-B的大部分特征。此外，在HeLa细胞中稳定表达的ApAurora可以替代人Aurora-A和Aurora-B，当其中一种被RNAi还原时。因此，在海星卵和HeLa细胞中，单个ApAurora同时具有Aurora-A和Aurora-B的特性。结合系统发育分析表明，ApAurora与所有类型的脊椎动物极光和非脊椎动物后口动物的单一极光形成一个分支，我们的观察结果支持这样的观点，即在非脊椎动物后口动物中发现的单一Aurora代表了产生各种脊椎动物极光的祖先。因此，这项研究为系统发育考量提供了功能证据。
Aurora, an essential mitotic kinase, is highly conserved during evolution. Most vertebrates have at least two Aurora kinases, Aurora-A and Aurora-B, which have distinct functions in the centrosome-spindle and inner centromere-midbody, respectively. However, some non-vertebrate deuterostomes have only a single Aurora. It remains to be verified whether the single Aurora performs the same functions as vertebrate Auroras A and B combined. We have isolated a cDNA of a single Aurora (ApAurora) from the echinoderm starfish, Asterina pectinifera, and show that ApAurora displays most features of both Aurora-A and Aurora-B in starfish oocytes and early embryos. Furthermore, ApAurora that is stably expressed in HeLa cells can substitute for both human Aurora-A and Aurora-B when either is reduced by RNAi. A single ApAurora thus has properties of both Aurora-A and Aurora-B in starfish eggs and HeLa cells. Together with phylogenetic analysis indicating that ApAurora forms a clade with all types of vertebrate Auroras and single Auroras of non-vertebrate deuterostomes, our observations support the idea that the single Aurora found in non-vertebrate deuterostomes represents the ancestor that gave rise to various types of vertebrate Auroras. This study thus provides functional evidence for phylogenetic considerations.