RNA and RNA binding proteins participate in early stages of cell spreading through spreading initiation centers
RNA and RNA binding proteins participate in early stages of cell spreading through spreading initiation centers
复制标题
DOI：
10.1016/s0092-8674(04)00456-8
复制
发表时间：
2004-05-28
期刊：
影响因子：
64.5
通讯作者：
Mann, M
中科院分区：
文献类型：
--
作者：
de Hoog, CL;Foster, LJ;Mann, M
局灶性粘连是在细胞-基质接触部位形成的专门的附着和信号中心。我们采用了一种基于定量质谱的方法，称为SILAC，以确定和定量蛋白质相互作用的附着依赖性的方式与黏着斑蛋白。随后的共聚焦显微镜发现了一个以前未描述的结构，我们称之为扩散起始中心（SIC），仅存在于细胞扩散的早期阶段。SICs含有粘着斑标记，似乎被肌动蛋白鞘包围，并且令人惊讶的是，含有许多RNA结合蛋白、核糖体RNA和可能的其他RNA。干扰FUS/TLS、hnRNP K和hnRNP E1的功能会导致扩散增加。铺展起始中心是不同于粘着斑的核糖核蛋白复合物，并证明了RNA和RNA结合蛋白在细胞铺展起始中的作用。
Focal adhesions are specialized attachment and signaling centers that form at sites of cell-matrix contacts. We employed a quantitative mass spectrometry-based method called SILAC to identify and quantify proteins interacting in an attachment-dependent manner with focal adhesion proteins. Subsequent confocal microscopy revealed a previously undescribed structure, which we have termed a spreading initiation center (SIC), existing only in early stages of cell spreading. SICs contain focal adhesion markers, appear to be surrounded by an actin sheath, and, surprisingly, contain numerous RNA binding proteins, ribosomal RNA, and perhaps other RNAs. Interfering with the function of FUS/TLS, hnRNP K, and hnRNP E1 results in increased spreading. Spreading initiation centers are ribonucleoprotein complexes distinct from focal adhesions and demonstrate a role for RNA and RNA binding proteins in the initiation of cell spreading.