A MULTIFUNCTIONAL GENE (TETR) CONTROLS TN10-ENCODED TETRACYCLINE RESISTANCE
A MULTIFUNCTIONAL GENE (TETR) CONTROLS TN10-ENCODED TETRACYCLINE RESISTANCE
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DOI：
10.1128/jb.150.2.633-642.1982
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发表时间：
1982-01-01
影响因子：
3.2
通讯作者：
MULLER, W
中科院分区：
文献类型：
--
作者：
BECK, CF;MUTZEL, R;MULLER, W
通过基因融合和克隆相结合的方法对[大肠杆菌]转座子Tn10的四环素抗性调节基因(tetR)进行了分析。该基因位于 Tn10 中心附近的 695 个碱基对的 HincII DNA 片段上。转录方向与编码 TetA 蛋白的邻近基因 tetA 的转录方向相反。 tetR 基因的基因产物（TetR 蛋白）的分子量为 23,000。 tetR-lacZ基因融合体编码膜结合的融合β-半乳糖苷酶，表明TetR蛋白本身是膜相关的。 tetR缺陷的突变体会导致组成型四环素耐药，但耐药水平降低。 tetR基因的表达由四环素诱导；在没有四环素的情况下，TetR 蛋白会关闭自身的合成。
The tetracycline resistance regulatory gene (tetR) of [Escherichia coli] transposon Tn10 was analyzed by a combination of methods involving gene fusion and cloning. This gene is located on a 695-base pair HincII DNA fragment near the center of Tn10. The direction of transcription is opposite to that of neighboring gene tetA, which encodes the TetA protein. The gene product of the tetR gene (the TetR protein) has a MW of 23,000. tetR-lacZ gene fusions encode fusion .beta.-galactosidases that are membrane bound, indicating that the TetR protein itself is membrane associated. Mutants defective in tetR result in constitutive tetracycline resistance, but the level of resistance is reduced. Expression of the tetR gene is induced by tetracycline; in the absence of tetracycline, the TetR protein turns off its own synthesis.