Cell adhesive and growth behavior on electrospun nanofibrous scaffolds by designed multifunctional composites
Cell adhesive and growth behavior on electrospun nanofibrous scaffolds by designed multifunctional composites
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设计的多功能复合材料在电纺纳米纤维支架上的细胞粘附和生长行为
DOI：
10.1016/j.colsurfb.2010.12.005
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发表时间：
2011-05-01
影响因子：
5.8
通讯作者：
Feng, Xi-Zeng
中科院分区：
文献类型：
--
作者：
Cao, Ding;Wu, Yi-Pan;Feng, Xi-Zeng
采用静电纺丝技术制备了聚L-乳酸（PLLA）与胶原（科尔）或羟基磷灰石（HA）或两者共混的纳米结构组织工程生物复合支架。通过扫描电子显微镜（SEM）、X射线光电子能谱（XPS）、水接触角（WCA）、傅里叶变换红外（FUR）和拉伸试验对静电纺丝支架的形貌、化学和拉伸性能进行表征。PLLA和胶原或（和）HA的直径范围为200-700 nm的电纺生物复合材料支架模拟具有良好互连的孔网络结构的细胞外基质（ECM）的纳米级结构。胶原蛋白的存在增加了支架的柔韧性，并增强了细胞的附着和增殖，而HA改善了支架的拉伸性能。采用DAPI核染色法和FDA/PI双染色法评价支架的生物相容性和接触细胞的存活率。结果支持293 T细胞在复合支架上生长良好的结论。与纯PLLA支架相比，在复合材料上观察到更大的活细胞密度，尤其是PLLA/HA/胶原支架。(C)2010爱思唯尔有限公司版权所有。
Nanostructured biocomposite scaffolds of poly(L-lactide) (PLLA) blended with collagen (coll) or hydroxyapatite (HA), or both for tissue engineering application, were fabricated by electrospinning. The electrospun scaffolds were characterized for the morphology, chemical and tensile properties by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), water contact angle (WCA), Fourier transform infrared (FUR) measurement, and tensile testing. Electrospun biocomposite scaffolds of PLLA and collagen or (and) HA in the diameter range of 200-700 nm mimic the nanoscale structure of the extracellular matrix (ECM) with a well-interconnection pore network structure. The presence of collagen in the scaffolds increased their hydrophility, and enhanced cell attachment and proliferation, while HA improved the tensile properties of the scaffolds. The biocompatibility of the electrospun scaffolds and the viability of contacting cells were evaluated by 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) nuclear staining and by fluorescein diacetate (FDA) and propidium iodide (PI) double staining methods. The results support the conclusion that 293T cells grew well on composite scaffolds. Compared with pure PLLA scaffolds a greater density of viable cells was seen on the composites, especially the PLLA/HA/collagen scaffolds. (C) 2010 Elsevier B.V. All rights reserved.