Morphine sulfate inhibits hypoxiainduced vascular endothelial growth factor expression in endothelial cells and cardiac myocytes
Morphine sulfate inhibits hypoxiainduced vascular endothelial growth factor expression in endothelial cells and cardiac myocytes
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DOI：
10.1006/jmcc.2001.1480
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发表时间：
2001-12-01
影响因子：
5
通讯作者：
Roy, S
中科院分区：
文献类型：
--
作者：
Balasubramanian, S;Ramakrishnan, S;Roy, S
血管内皮生长因子（VEGF）是一种血管生成有丝分裂原，对内皮细胞具有特异性。缺氧诱导的血管内皮细胞和心肌细胞中的VEGF分别导致自分泌和旁分泌刺激。在心肌缺血期间，VEGF在内皮和心肌中上调，并且可能介导血管生成。硫酸吗啡常用于缓解急性心肌梗死患者的疼痛。我们研究了硫酸吗啡对缺氧条件下培养的内皮细胞和心肌细胞VEGF表达的影响。酶联免疫吸附试验显示，硫酸吗啡显著抑制缺氧诱导的小鼠心脏微血管内皮细胞（SMHEC 4）、人脐静脉内皮细胞（HUVEC）和大鼠心肌细胞VEGF的表达（P
Vascular endothelial growth factor (VEGF) is an angiogenic mitogen, specific for endothelial cells. Hypoxia-induced VEGF in endothelial cells and cardiomyocytes leads to autocrine and paracrine stimulation, respectively. During myocardial ischemia, VEGF is upregulated in the endothelium and myocardium, and may mediate angiogenesis. Morphine sulfate is commonly used in pain relief for patients with acute myocardial infarction. We investigated the effect of morphine sulfate on VEGF expression in cultured endothelial cells and cardiac myocytes subjected to hypoxia. Enzyme-linked immunosorbent assays showed that morphine sulfate significantly inhibited hypoxia-induced VEGF expression in mouse heart microvascular endothelial cells (SMHEC4), primary cultures of human umbilical vein endothelial cells (HUVECs) and in primary cultures of rat cardiac myocytes (P