Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization
Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization
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DOI：
10.1007/978-1-60761-376-3_4
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Holzinger, Andreas
中科院分区：
文献类型：
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作者：
Holzinger, Andreas
Jasplakin是一种环缩肽，是一种常用的肌动蛋白丝聚合和稳定药物。这种物质最初是从海绵中分离出来的，现在可以合成，并已上市。这一点，连同jasplakin是膜可渗透的益处，使其成为细胞生物学中常用的工具，当必须实现肌动蛋白丝稳定化或聚合时。这可能是在形态发生，运动性，细胞器运动的研究中的情况下，或当细胞凋亡被诱导。它作为一种有效的抗癌药物的使用进行了讨论。对肌动蛋白丝的直接作用可能对高尔基体和膜筏蛋白组织产生进一步的影响。在这一章中，不同的荧光和透射电子显微镜方法的可视化jasplaklinearity的影响进行了说明。由于jasplakinidase和鬼笔环肽的竞争性结合能力使得通过荧光标记的鬼笔环肽检测肌动蛋白丝成为问题，因此在此给出了替代方案。
Jasplakinolide, a cyclo-depsipeptide is a commonly used actin filament polymerizing and stabilizing drug. The substance has originally been isolated from a marine sponge, and can now be synthesized and has become commercially available. This, together with the benefit that jasplakinolide is membrane permeable has made it a commonly used tool in cell biology, when actin filament stabilization or polymerization has to be achieved. This may either be the case in studies on morphogenesis, motility, organelle movement, or when apoptosis has to be induced. Its use as a potent anticancer drug is discussed. The direct action on actin filaments may have further consequences in golgi body and membrane raft protein organization. In this chapter, the visualization of jasplaklinolide effects by different fluorescent and transmission electron microscopic methods is described. As competitive binding capacities of jasplakinolide and phalloidin make the detection of actin filaments by fluorescently labeled phalloidin problematic, alternatives are given here.