Implanted hair-follicle-associated pluripotent (HAP) stem cells encapsulated in polyvinylidene fluoride membrane cylinders promote effective recovery of peripheral nerve injury
Implanted hair-follicle-associated pluripotent (HAP) stem cells encapsulated in polyvinylidene fluoride membrane cylinders promote effective recovery of peripheral nerve injury
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DOI：
10.1080/15384101.2017.1363941
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发表时间：
2017-01-01
期刊：
影响因子：
4.3
通讯作者：
Amoh, Yasuyuki
中科院分区：
文献类型：
--
作者：
Yamazaki, Aiko;Obara, Kohya;Amoh, Yasuyuki
毛囊相关多能（HAP）干细胞位于毛囊的隆突区域，表达干细胞标记物巢蛋白，并且已显示分化为神经细胞、神经胶质细胞、角质形成细胞、平滑肌细胞、心肌细胞和黑素细胞。移植的HAP干细胞可促进周围神经和脊髓损伤的恢复，并具有心脏再生的潜力。在本研究中，我们植入小鼠绿色荧光蛋白（GFP）表达HAP干细胞球封装在聚偏氟乙烯（PVDF）膜圆柱体到切断坐骨神经的免疫活性和免疫功能低下（裸）小鼠。植入8周后，免疫荧光染色显示HAP干细胞分化为神经元和神经胶质细胞。荧光显微镜显示，HAP干细胞毛球促进免疫活性小鼠和免疫缺陷小鼠坐骨神经的重新连接。苏木精-伊红（H&E）染色显示切断的坐骨神经已再生。定量行走分析表明，移植小鼠恢复了正常行走的能力。HAP干细胞容易从每个人获得，不形成肿瘤，并且可以冷冻保存而不丧失分化潜力。这些结果表明，HAP干细胞可能比iPS或ES细胞具有更大的再生医学潜力。
Hair follicle-associated-pluripotent (HAP) stem cells are located in the bulge area of the hair follicle, express the stem-cell marker, nestin, and have been shown to differentiate to nerve cells, glial cells, keratinocytes, smooth muscle cells, cardiac muscle cells, and melanocytes. Transplanted HAP stem cells promote the recovery of peripheral nerve and spinal cord injuries and have the potential for heart regeneration as well. In the present study, we implanted mouse green fluorescent protein (GFP)-expressing HAP stem-cell spheres encapsulated in polyvinylidene fluoride (PVDF)-membrane cylinders into the severed sciatic nerve of immunocompetent and immunocompromised (nude) mice. Eight weeks after implantation, immunofluorescence staining showed that the HAP stem cells differentiated into neurons and glial cells. Fluorescence microscopy showed that the HAP stem cell hair spheres promoted rejoining of the sciatic nerve of both immunocompetent and immunodeficient mice. Hematoxylin and eosin (H&E) staining showed that the severed scatic nerves had regenerated. Quantitative walking analysis showed that the transplanted mice recovered the ability to walk normally. HAP stem cells are readily accessible from everyone, do not form tumors, and can be cryopreserved without loss of differentiation potential. These results suggest that HAP stem cells may have greater potential than iPS or ES cells for regenerative medicine.