Induction by acid load of the maturation of prestalk cells in Dictyostelium discoideum
Induction by acid load of the maturation of prestalk cells in Dictyostelium discoideum
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酸负荷诱导盘基网柄菌前茎细胞成熟
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发表时间：
1988
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影响因子：
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通讯作者：
K. Inouye
中科院分区：
文献类型：
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作者：
K. Inouye
在细胞粘菌盘基网柄菌子实体构建过程中，前茎细胞以良好控制的方式变成成熟的茎细胞。为了鉴定茎细胞成熟的天然诱导剂，研究了已知在体外条件下诱导茎细胞分化的物质以及一些其他相关化合物在体内对迁移蛞蝓（子实体的前体结构）的影响。在这些物质中，添加弱酸如CO2，以及添加后去除弱碱如NH3，显着诱导了蛞蝓中前柄细胞的原位成熟。另一方面，质膜质子泵抑制剂并不能有效诱导完整蛞蝓中前柄细胞的成熟。分化诱导因子（DIF）是茎前分化的内源性诱导剂，当应用于完整的蛞蝓时，它似乎是茎细胞成熟的更差的诱导剂。这些物质诱导茎细胞成熟的活性与其对前茎细胞细胞质pH（pHi）的影响表现出良好的相关性； pHi 下降越大，对茎细胞成熟的诱导越强，表明前茎细胞的成熟需要 pHi 下降。基于这些结果，有人提出，由 DIF 诱导的茎细胞分化在正常发育过程中被阻止 pHi 降低的试剂阻断。
During the process of fruiting body construction in the cellular slime mould Dictyostelium discoideum, prestalk cells become mature stalk cells in a well-controlled manner. To identify the natural inducer of stalk cell maturation, substances known to induce stalk cell differentiation under in vitro conditions, and some other related compounds, were examined for their effects in vivo on migrating slugs, the precursor structures of the fruiting bodies. Among these substances, addition of weak acids such as CO2, and addition followed by removal of weak bases such as NH3, strikingly induced the maturation of prestalk cells in situ in slugs. On the other hand, inhibitors of the plasma membrane proton pump did not efficiently induce the maturation of prestalk cells in intact slugs. Differentiation inducing factor (DIF), an endogenous inducer of prestalk differentiation, seemed to be an even poorer inducer of stalk cell maturation when applied to intact slugs. The activities of these substances in inducing stalk cell maturation showed a good correlation with their effects on the cytoplasmic pH (pHi) of prestalk cells; the larger the pHi drop, the stronger the induction of stalk cell maturation, suggesting a requirement for a pHi decrease for the maturation of prestalk cells. Based on these results, it was proposed that stalk cell differentiation, which is induced by DIF, is blocked halfway during normal development by (an) agent(s) that prevent(s) the decrease in pHi.