Expression of growth hormone-responsive serpin mRNAs in perinatal rat liver.
Expression of growth hormone-responsive serpin mRNAs in perinatal rat liver.
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生长激素反应性丝氨酸蛋白酶抑制剂 mRNA 在围产期大鼠肝脏中的表达。
DOI：
10.1152/ajpendo.1993.264.6.e973
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Bundy,MV
中科院分区：
文献类型：
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作者：
Berry,SA;Bergad,PL;Bundy,MV
控制胎儿生长的激素机制知之甚少，一般认为生长激素（GH）不会影响围产期生长。为了研究生长激素对围产期大鼠肝脏基因表达的影响，我们测定了几个生长激素反应和生长轴基因的RNA水平。从妊娠第19、20、21天和出生后第2天测量大鼠肝脏总RNA中的Spi 2.1、Spi 2.2、Spi 2.3、胰岛素样生长因子（IGF）I和II以及GH受体mRNA。Spi 2.1和2.3基因在妊娠第20天微弱表达，妊娠第21天为成人水平的6%和13 +/- 1%，妊娠第2天为成人水平的6%和31 +/- 6%。Spi 2.1基因5'侧翼区的脱氧核糖核酸酶I（DNase I）超敏感位点与GH反应一致，在妊娠第19天从肝脏提取的DNA中不存在，但在第20、21和2天存在，这表明该基因在第19天后具有转录能力，并且染色质易受DNA酶I消化的区域在前产后生活在所有测量日均存在低水平的GH受体mRNA（约为成人的10%）。第19或20天胎儿样本中IGF-I mRNA低于可定量水平，第21天仅为成人水平的2.7 ± 0.1%。第2天的水平为成人的9.6 +/- 1.9%。IGF-II mRNA在此期间基本恒定，在妊娠第21天略有增加。（250字处删节）
Hormonal mechanisms controlling growth of the fetus are poorly understood, and generally growth hormone (GH) is not thought to influence perinatal growth. To examine the influence of GH in the expression of genes in perinatal rat liver, we measured RNA levels of several GH responsive and growth axis genes. Spi 2.1, Spi 2.2, Spi 2.3, insulin-like growth factors (IGF) I and II, and GH receptor mRNAs were measured in rat liver total RNA from gestational days 19, 20, 21, and postnatal day 2. Spi 2.1 and 2.3 genes were faintly expressed on day 20, 6% and 13 +/- 1% of adult levels on gestation day 21, and 6% and 31 +/- 6% of adult levels on day 2. Deoxyribonuclease I (DNase I)-hypersensitive sites in the 5' flanking region of the Spi 2.1 gene, which are concordant with GH response, were not present in DNA extracted from livers at gestation day 19 but were present at days 20, 21, and 2, suggesting the gene is transcriptionally competent after day 19 and that the areas of chromatin vulnerable to DNase I digestion are the same in pre- and postnatal life. Low levels of GH receptor mRNAs (approximately 10% of adult) were present on all measured days. IGF-I mRNA was below quantitatable levels in day 19 or 20 fetal samples and was only 2.7 +/- 0.1% of adult levels on day 21. Levels on day 2 were 9.6 +/- 1.9% of adult. IGF-II mRNA was essentially constant throughout this period, with a minimal increase at day 21 of gestation.(ABSTRACT TRUNCATED AT 250 WORDS)