PRETERM LABOR - STIMULATION OF ARACHIDONIC-ACID METABOLISM IN HUMAN AMNION CELLS BY BACTERIAL PRODUCTS
PRETERM LABOR - STIMULATION OF ARACHIDONIC-ACID METABOLISM IN HUMAN AMNION CELLS BY BACTERIAL PRODUCTS
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DOI：
10.1016/0002-9378(87)90070-6
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发表时间：
1987-03-01
影响因子：
9.8
通讯作者：
ELDER, MG
中科院分区：
文献类型：
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作者：
BENNETT, PR;ROSE, MP;ELDER, MG
早产和感染之间有很强的关联。一些潜在的致病细菌具有磷脂酶活性，并且已经表明从这些生物体释放磷脂酶可能增加羊膜细胞中的前列腺素E2合成，从而引发早产。在这项研究中，我们建立了单层羊膜细胞培养的组织收集在择期剖宫产在足月前分娩。用氚化花生四烯酸预标记细胞，然后在加入2%、5%或10%（体积/体积）过滤的培养基后进一步孵育，其中组B β-溶血性链球菌、草绿色链球菌、大肠杆菌、脆弱拟杆菌或乳酸杆菌一直在生长。氚化花生四烯酸及其代谢产物释放的羊膜细胞在这些或控制孵育提取培养基和分离的高效液相色谱法。除了乳酸菌外，每种微生物的条件培养基的添加引起总体花生四烯酸代谢的增加。与对照组相比，环氧合酶与脂氧合酶代谢的比例增加，特别是前列腺素E2的产生增加。添加过滤细菌培养基后羊膜细胞中花生四烯酸代谢的概况与自然分娩后培养的羊膜细胞中获得的相似。我们认为，生殖道的异常细菌定植可能导致羊膜细胞中花生四烯酸代谢的增加，前列腺素E2的产生增加，从而引发早产。
There is a strong association between preterm labor and infection. Some potentially pathogenic bacteria have phospholipase activity, and it has been suggested that release of phospholipase from these organism may increase prostaglandin E2 synthesis in amnion cells and hence initiate preterm labor. In this study we established monolayer amnion cell cultures from tissue collected at elective cesarean section at term before labor. Cells were prelabeled with tritiated arachidonic acid and then further incubated after addition of 2%, 5%, or 10% (vol/vol) filtered medium in which either group B .beta.-hemolytic streptococcus, Streptococcus viridans, Escherichia coli, Bacteroides fragilis, or Lactobacillus had been growing. Tritiated arachidonic acid and its metabolites released by the amnion cells in these or control incubates were extracted from culture medium and separated by high-performance liquid chromatography. Addition of conditioned medium from each of the organisms with the exception of Lactobacillus caused an increase in overall arachidonic acid metabolism. There was an increase in the ratio of cyclooxygenase to lipoxygenase metabolism and in prostaglandin E2 production in particular when compared to controls. The profile of arachidonic acid metabolism in amnion cells following addition of filtered bacterial medium resembled that obtained from amnion cells cultured following spontaneous labor. We suggest that abnormal bacterial colonization of the genital tract may lead to an increase in arachidonic acid metabolism in amnion cells with an increase in prostaglandin E2 production and the consequent initiation of preterm labor.