Tortoise DNA detection from highly processed tortoise shell using SINE element
Tortoise DNA detection from highly processed tortoise shell using SINE element
复制标题
使用 SINE 元件从高度加工的龟甲中检测龟 DNA
DOI：
10.38212/2224-6614.2284
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发表时间：
2020-07
影响因子：
3.6
通讯作者：
周祥山
中科院分区：
文献类型：
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作者：
Bang-Ce Ye;Jinhua You;Yuanxing Zhang;Pin Lv;周祥山
龟甲胶是一种珍贵的传统中药，是经过深加工的龟甲胶，含有微量的严重降解的DNA。在这项工作中，首次开发了一种简单快速的方法，用于从替代品中鉴别高度加工的药物，这些替代品被欺骗性地标记为龟甲胶，但来自其他动物，如马，牛或猪的组织。该方法基于聚合酶链反应（PCR）扩增乌龟特异的Pol Ⅲ/SINE元件，可用于检测仅含0.1%龟甲胶的胶液中的乌龟DNA。还根据它们在PCR中的性能对用于测定的五种不同聚合酶进行了比较。最后，将该方法应用于6种市售龟甲胶产品中的乌龟DNA检测，其中1种产品检测结果为阴性，表明该产品中不含或含有少于0.1%的龟甲。
Colla carapax et plastrum testudinis is one of the most valuable traditional Chinese medicine referring to highly processed tortoise shell glue which contains trace amount of severely degraded DNA. In this work, a simple and fast methodology was first developed for the identification of highly processed medicine from substitutions which were labeled fraudulently as tortoise shell glue but derived from tissues of other animals such as horse, cattle or pig. The method is based on polymerase chain reaction (PCR) amplification of tortoise-specific Pol Ⅲ/SINE element and allows the detection of tortoise DNA in glue mixtures, which contain only 0.1% colla carapax et plastrum testudinis. A comparison of five various polymerases for the assay was also conducted, based on their performances in PCR. Finally, the method was applied for the detection of tortoise DNA from six commercial tortoise shell glue products; one of them was tested negative, indicating none or less than 0.1% tortoise shell in there.