Genetic parameters of milk ELISA scores for Johne's disease
Genetic parameters of milk ELISA scores for Johne's disease
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DOI：
10.3168/jds.2009-2625
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发表时间：
2010-04-01
影响因子：
3.5
通讯作者：
Heins, B. J.
中科院分区：
文献类型：
--
作者：
Attalla, S. A.;Seykora, A. J.;Heins, B. J.
本研究的目的是估计禽分枝杆菌SSP抗体应答的遗传参数。使用常规收集的明尼苏达州奶牛群改良牛奶进行副结核病的酶联免疫吸附试验。在所有编辑之后，来自282个Johne阳性牛群中的21,514头荷斯坦奶牛的25,809个测试可用于分析。Johne的检测结果被分析为二元性状(阳性或阴性)和线性性状，即转化后的ELISA光密度[ln(OD)]。模型中显著的固定效应是试验日期的年龄、乳汁中的天数和实验室阴性对照；随机效应是羊群试验日期、动物效应和永久环境效应。转化后的ELISA光密度随试验日龄和乳汁中天数的增加而增加。遗传力估计在0.065到0.095之间。用母体效应解释的变异百分比在1.3%到2.29%之间。重复性从0.380到0.433不等。在分析中，154头至少有30头母牛的公牛的Ln(OD)的父本解决方案与它们的美国农业部预测的传递能力之间的统计相关性如下：脂肪产量-0.199；蛋白质产量-0.179；生产寿命-0.292；净功-0.339。这些相关性表明，选择生产生活或净值也将提高对约翰氏病的抵抗力。
The objective of this study was to estimate genetic parameters of antibody response to Mycobacterium avium ssp. paratuberculosis using routinely collected Minnesota Dairy Herd Improvement milk ELISA tests. After all edits, 25,809 tests from 21,514 Holstein cows in 282 Johne's positive herds were available for analysis. The Johne's test results were analyzed both as a binary trait (positive or negative) and as a linear trait as the transformed ELISA optical density [ln(OD)]. Significant fixed effects in the model were age at test date, days in milk, and laboratory negative control; random effects were herd test date, animal effect, and permanent environment effect. Transformed ELISA optical density increased with age at test day and days in milk. Heritability estimates ranged from 0.065 to 0.095. Percentage of variation explained by maternal effects ranged from 1.3 to 2.29%. Repeatabilities ranged from 0.380 to 0.433. Statistically significant correlations between the sire solutions for ln(OD) for 154 bulls with at least 30 daughters in the analysis and their USDA predicted transmitting abilities were as follows: fat yield, -0.199; protein yield, -0.179; productive life, -0.292; and Net Merit, -0.339. These correlations suggest that selection for productive life or Net Merit also will improve resistance to Johne's disease.