Cross-reactivity between storage and dust mites and between mites and shrimp
Cross-reactivity between storage and dust mites and between mites and shrimp
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DOI：
10.1007/s10493-008-9199-x
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发表时间：
2009-02-01
影响因子：
2.2
通讯作者：
Sharra, Denada
中科院分区：
文献类型：
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作者：
Arlian, Larry G.;Morgan, Marjorie S.;Sharra, Denada
许多患者对多种储存和室内尘螨敏感。目前尚不清楚这是因为患者对特定种类的螨虫过敏原有多重敏感性，还是这些螨虫有许多交叉反应的过敏原。我们的目的是利用交叉免疫电泳（CIE）、交叉放射免疫电泳（CRIE）、免疫印迹和ELISA进一步确定几种储存尘螨和室内尘螨之间的交叉致敏性。CIE和CRIE反应表明，在螨过敏患者的血清池中，储存螨具有两个交叉抗原分子，其中一个结合IgE。针对不同螨种构建的抗血清抗体能够识别许多其他螨种的SDS-PAGE分解蛋白，这表明可能存在其他交叉反应性过敏原。在患者血清中，IgE与许多不同的蛋白质结合，但很少有IgE与不同螨种中具有共同分子量的蛋白质结合，这表明主要是物种特异性过敏原。针对每种螨类构建的抗血清在虾提取物中沉淀出一种蛋白，该蛋白结合血清池中的抗der p10（原肌球蛋白）和IgE。Anti-Der p10与虾原肌球蛋白结合较强，与螨蛋白结合较弱。ELISA结果显示，螨提取物中原肌球蛋白含量极低。与虾和蜗牛相比，所调查的尘螨和尘螨主要含有物种特异性过敏原和极少量的泛过敏原原肌球蛋白。
Many patients have sensitivities to multiple species of storage and house dust mites. It is not clear if this is because patients have multiple sensitivities to species-specific mite allergens or if these mites share many cross-reacting allergens. Our objective was to further define the cross-allergenicity between several species of storage and house dust mites using crossed-immunoelectrophoresis (CIE), crossed-radioimmunoelectrophoresis (CRIE), immunoblotting, and ELISA. CIE and CRIE reactions revealed that storage mites shared two cross-antigenic molecules and one of these bound IgE in a serum pool from mite allergic patients. Antibody in anti-sera built to each species of mite recognized many SDS-PAGE resolved proteins of other mite species and this suggested the potential for other cross-reactive allergens. Among patient sera, IgE bound to many different proteins but few had IgE that bound to a protein with common molecular weights across the mite species and this suggested mostly species-specific allergens. Antiserum built to each mite species precipitated one protein in shrimp extracts that bound anti-Der p 10 (tropomyosin) and IgE in the serum pool. Anti-Der p 10 showed strong binding to shrimp tropomyosin but very little to any of the mite proteins. ELISA showed the mite extracts contained very little tropomyosin. The storage and dust mites investigated contain mostly species-specific allergens and very small amounts of the pan-allergen tropomyosin compared to shrimp and snail.