A non-flagellated, predatory soil bacterium reprograms a chemosensory system to control antifungal antibiotic production via cyclicdi-GMPsignalling
A non-flagellated, predatory soil bacterium reprograms a chemosensory system to control antifungal antibiotic production via cyclicdi-GMPsignalling
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一种无鞭毛的捕食性土壤细菌重新编程化学感应系统，通过环二 GMP 信号控制抗真菌抗生素的产生
DOI：
10.1111/1462-2920.15191
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发表时间：
2020-09-09
影响因子：
5.1
通讯作者：
Qian, Guoliang
中科院分区：
文献类型：
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作者：
Xu, Kangwen;Shen, Danyu;Qian, Guoliang
产酶溶杆菌是一种无鞭毛的土壤变形杆菌，分泌一种可扩散的抗生素，称为热稳定抗真菌因子（HSAF），以杀死附近的真菌作为食物。模型菌株OH 11的基因组编码同源Wsp系统，其通常由有鞭毛的细菌部署以通过c-di-GMP-FleQ复合物实现鞭毛依赖性输出，其中c-di-GMP是普遍存在的二核苷酸第二信使，FleQ是转录因子（TF）。在这里，我们表明，Wsp系统在非鞭毛OH 11参与一个独特的c-di-GMP依赖的信号通路，并形成WspR-CdgL二元复合物，以改变HSAF的生产，其中WspR和CdgL作为一个c-di-GMP二鸟苷酸环化酶（DGC）和非TF结合蛋白。我们发现WspR的磷酸化激活其DGC活性并增强c-di-GMP产生，同时抑制HSAF生物合成。WspR的磷酸化也在减弱WspR-CdgL结合和HSAF产生中起关键作用。有趣的是，c-di-GMP与CdgL的结合似乎并不诱导WspR-CdgL复合物的解离。这些观察结果，沿着我们早期的发现，使我们提出了一个模型，其中L. enzymogenes通过c-di-GMP信号转导重新编程Wsp系统，以调节HSAF生物合成，从而有利于生态适应。
Lysobacter enzymogenesis a non-flagellated, soil proteobacterium that secretes a diffusible antibiotic known as heat-stable antifungal factor (HSAF) to kill nearby fungi for food. The genome of the model strain OH11 encodes a homologous Wsp system, which is generally deployed by flagellated bacteria to achieve flagella-dependent outputs via a c-di-GMP-FleQ complex, in which c-di-GMP is a ubiquitous dinucleotide second messenger and FleQ is a transcription factor (TF). Here, we show that the Wsp system in the non-flagellated OH11 participates in a unique c-di-GMP-dependent signalling pathway and forms a WspR-CdgL binary complex to alter HSAF production, in which WspR and CdgL act as a c-di-GMP diguanylate cyclase (DGC) and a non-TF binding protein respectively. We found that the phosphorylation of WspR activates its DGC activity and enhances c-di-GMP production while inhibiting HSAF biosynthesis. The phosphorylation of WspR also plays a key role in weakening WspR-CdgL binding and HSAF generation. Interestingly, c-di-GMP binding to CdgL did not seem to induce the disassociation of the WspR-CdgL complex. These observations, along with our earlier findings, lead us to propose a model in whichL.enzymogenesre-programs the Wsp system via c-di-GMP signalling to regulate HSAF biosynthesis for the benefit of ecological adaptation.