Distributional Characteristics of Virulence-associated Gene of Streptococcus suis Strains Isolated from China
Distributional Characteristics of Virulence-associated Gene of Streptococcus suis Strains Isolated from China
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发表时间：
2006
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通讯作者：
Lu Cheng-ping
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作者：
Lu Cheng-ping
为了解猪链球菌（Streptococcus suis，SS）毒力相关因子的分布，采用PCR方法检测了7个SS毒力相关因子，即谷氨酸脱氢酶基因（glutamate dehydrogenase gene，gdh）、荚膜多糖基因（capsular polysaccharides gene，cps）、胞外因子基因（extracellular factor gene，epf）、溶菌酶释放蛋白基因（mrp）、猪溶血素基因（sulysin gene，sly）纤连蛋白结合蛋白基因（fpbs）和毒力相关序列orf2。29个来自中国的强毒株，包括25个SS2，1个SS1，25株中有24株为cps +/gdh +/epf +/mrp +/sly +/fbps +/orf2+阳性，1株为cps +/gdh +/epf-/mrp +/sly +/fbps +/orf2+阳性，2株为cps-/gdh +/epf-/mrp-/sly +/fbps-/orf2+阳性，2株为cps-/gdh +/epf-/mrp-/sly +/fbps-/orf2+阳性，2株为cps-/gdh +/epf-/mrp-/sly +/fbps-/orf2+阳性，1株为cps-/gdh +/epf-/mrp-/sly +/fbps-/orf2+阳性，2株为cps-/gdh +/epf-/mrp-/sly +/fbps-/orf2+阳性结果表明，我国猪链球菌强毒株毒力相关因子的分布与欧洲不同，我国猪链球菌2型的流行株为7种毒力相关因子均为强毒株，其毒力相关因子的分布与欧洲猪链球菌不同，我国猪链球菌2型的流行株为7种毒力相关因子均为强毒株。
To investigate the distribution of virulence associate factors of Streptococcus suis(SS),seven virulence factors were detected by PCR amplification,namely the glutamate dehydrogenase gene(gdh),the capsular polysaccharides gene(cps),the extracellular factor gene(epf),the muramidase-released protein gene(mrp),the suilysin gene(sly),the fibronectin-binding protein gene(fpbs) and the virulence-associated sequence orf2.Twenty-nine virulent isolates from China including twenty-five SS2,one SS1,one SS7 and two SS9,were detected by PCR method.96%(24/25) were positive of cps+/gdh+/epf+/mrp+/sly+/fbps+/orf2+ and only one virulent SS2 isolate was cps+/gdh+/epf-/mrp+/sly+/fbps+/orf2+.Two strains of SS1 and SS7 were cps-/gdh+/epf-/mrp-/sly+/fbps-/orf2+,while,two strains of SS9 were cps-/gdh+/epf-/mrp-/sly-/fbps-/orf2+.The results showed the distribution of virulent associate factors of virulent streptococcus suis in China was different from isolates of Europe.The prevalent strains of SS2 in China were highly virulent stains which contain all seven virulence factors.