A rapid analysis of copepod feeding using FlowCAM
A rapid analysis of copepod feeding using FlowCAM
复制标题
DOI：
10.1093/plankt/fbm108
复制
发表时间：
2008-03-01
影响因子：
2.1
通讯作者：
Saito, Hiroaki
中科院分区：
文献类型：
--
作者：
Ide, Keiichiro;Takahashi, Kazutaka;Saito, Hiroaki
通过与传统显微分析方法的比较，本研究探讨了使用新型浮游生物图像分析仪FlowCAM快速分析桡足类摄食的有效性和可靠性。我们对Oyashio地区的两种桡足类动物进行了瓶式孵化实验，并分析了FlowCAM孵化前后的猎物丰度。根据颗粒的体积比荧光强度，FlowCAM成功地区分了浮游动物和浮游植物，并允许充分评估以浮游动物和浮游植物为食的桡足类动物。一个浮游生物样本的分析时间约为10分钟，不到显微计数所需时间的十分之一。FlowCAM被认为是一种快速分析桡足类摄食的有效工具，特别是在杂食性研究中。
This study addressed the usefulness and reliability of using a new plankton image analyzer, FlowCAM, for rapid analysis of copepod feeding by comparison with the conventional microscopic analysis. We carried out bottle incubation experiments with two copepod species in the Oyashio region and analyzed the prey abundance prior to and after the incubation with a FlowCAM. From the volume-specific fluorescence intensity of particles, the FlowCAM successfully distinguished between zooplankton and phytoplankton and allowed an adequate evaluation of the copepod feeding on zooplankton and phytoplankton. The analysis time for one plankton sample was about 10 min, which was less than one-tenth of the time required for microscopic enumeration. The FlowCAM is considered to be an efficient tool for rapid analysis of copepod feeding particularly in studies of omnivory.