The diagnostic value of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) measurement in the sera of gastric cancer patients
The diagnostic value of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) measurement in the sera of gastric cancer patients
复制标题
DOI：
10.1007/s10238-010-0097-2
复制
发表时间：
2010-12-01
影响因子：
4.6
通讯作者：
Szmitkowski, Maciej
中科院分区：
文献类型：
--
作者：
Jelski, Wojciech;Orywal, Karolina;Szmitkowski, Maciej
胃癌细胞（GC）中存在乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）。此外，癌组织中总ADH和IV类同工酶的活性显着高于健康粘膜。癌细胞中这些酶的活性可能反映在血清中，因此有助于胃癌的诊断。本研究的目的是探讨 ADH 和 ALDH 作为胃癌肿瘤标志物的潜在作用。我们定义了诊断敏感性、特异性、阳性和阴性结果的预测值，以及测试酶的受试者操作特征 (ROC) 曲线。血清样本取自 168 名治疗前的胃癌患者和 168 名对照受试者。总 ADH 活性以及 III 类和 IV 类同工酶通过光度法测量，但 ALDH 活性以及 ADH I 和 II 通过荧光法以及特定类荧光底物进行测量。与对照组相比，胃癌患者血清中ADH IV同工酶活性和ADH总量显着升高。 ADH IV 的诊断敏感性为 73%，特异性为 79%，阳性和阴性预测值分别为 81% 和 72%。 ADH IV 的 ROC 曲线下面积为 0.67。结果表明 ADH IV 作为胃癌标志物的潜在作用。
Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are present in gastric cancer cells (GC). Moreover, the activity of total ADH and class IV isoenzymes is significantly higher in cancer tissue than in healthy mucosa. The activity of these enzymes in cancer cells is probably reflected in the sera and could thus be helpful for diagnostics of gastric cancer. The aim of this study was to investigate a potential role of ADH and ALDH as tumor markers for gastric cancer. We defined diagnostic sensitivity, specificity, predictive value for positive and negative results, and receiver-operating characteristics (ROC) curve for tested enzymes. Serum samples were taken from 168 patients with gastric cancer before treatment and from 168 control subjects. Total ADH activity and class III and IV isoenzymes were measured by photometric but ALDH activity and ADH I and II by the fluorometric method, with class-specific fluorogenic substrates. There was significant increase in the activity of ADH IV isoenzyme and ADH total in the sera of gastric cancer patients compared to the control. The diagnostic sensitivity for ADH IV was 73%, specificity 79%, positive and negative predictive values were 81 and 72% respectively. Area under ROC curve for ADH IV was 0.67. The results suggest a potential role for ADH IV as marker of gastric cancer.