Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction
Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction
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聚合酶链式反应检测 HTLV-I 血清阳性母亲所生血清阴性婴儿的 HTLV-I 基因组
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发表时间：
1989
期刊：
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通讯作者：
K. Sugamura
中科院分区：
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作者：
S. Saito;Y. Ando;K. Furuki;K. Kakimoto;T. Tanigawa;I. Moriyama;M. Ichijo;Masataka Nakamura;K. Ohtani;K. Sugamura
我们应用聚合酶链反应（PCR）方法检测HTLV-I血清阳性母亲所生的血清阴性婴儿外周血淋巴细胞中人T细胞白血病病毒I型（HTLV-I）原病毒的gag、env和pX序列。在 22 名受试者中，有 5 名受试者被发现含有 HTLV-I 原病毒基因组。通过常规抗体/抗原检测方法，5例病例中的2例不仅抗HTLV-I抗体呈阴性，培养淋巴细胞上的病毒抗原也呈阴性。这些结果表明 PCR 作为一种简单且高度灵敏的检测 HTLV-I 感染的方法具有很大的用途。
We applied the polymerase chain reaction (PCR) method to detect gag, env and pX sequences of human T cell leukemia virus type I (HTLV‐I) provirus in peripheral blood lymphocytes of seronega‐tive infants born to HTLV‐I seropositive mothers. Out of 22, five subjects were found to contain the HTLV‐I provirus genome. Two of the five cases were judged to be negative for not only anti‐HTLV‐I antibodies but also the viral antigens on cultivated lymphocytes by the conventional antibody/antigen detection methods. These results indicate that PCR is of great use as a simple and highly sensitive method detect HTLV‐I infection.