Biogenic synthesis of silver nanoparticles using Brazilian propolis
Biogenic synthesis of silver nanoparticles using Brazilian propolis
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DOI：
10.1002/btpr.2888
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发表时间：
2019-08-16
影响因子：
2.9
通讯作者：
Dornelas, Camila B.
中科院分区：
文献类型：
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作者：
Barbosa, Valcilaine T.;Souza, Joyelanne K. C.;Dornelas, Camila B.
由于银纳米粒子具有还原性、稳定性和环境友好性等特点，生物法已被广泛应用于细菌、真菌、植物、真菌等材料的制备。考虑到巴西棕榈油的抗菌活性以及银纳米颗粒的广谱抗菌作用，本研究旨在描述使用巴西棕榈油合成银纳米颗粒（AgNP-P）并研究其抗菌活性。通过析因设计优化合成，选择最佳条件用于较小尺寸的颗粒。AgNP-P在紫外-可见光谱中在412 nm处表现出最大吸收，这表明其为球形形式并形成。动态光散射表明流体动力学尺寸为109 nm，多分散指数小于0.3，显示出良好的尺寸分布和稳定性。在其通过离心纯化后，显微镜分析证实了该形式，并显示在银纳米颗粒周围存在纳米颗粒。X射线衍射峰归因于金属银晶体结构的主平面;同时红外光谱证明了导致银还原的主要基团，其代表性与热分析表明的AgNP-P的22%相似。我们的产品显示出重要的抗微生物活性，表明如预期的那样，纳米粒子和银纳米粒子之间存在协同作用，并且有望成为用于感染的有效抗微生物产品。
Biological methods have been used to synthesize silver nanoparticles through materials such as bacteria, fungi, plants, and propolis due to their reducing properties, stabilizer role and environmentally friendly characteristic. Considering the antimicrobial activity of propolis as well as the broad-spectrum antibacterial effects of silver nanoparticles, this study aim to describe the use of Brazilian propolis to synthesize silver nanoparticles (AgNP-P) and investigate its antimicrobial activity. The synthesis was optimized by factorial design, choosing the best conditions for smaller size particles. AgNP-P demonstrated a maximum absorbance at 412 nm in ultraviolet-visible spectra, which indicated a spherical format and its formation. Dynamic light scattering demonstrated a hydrodynamic size of 109 nm and polydispersity index less than 0.3, showing a good size distribution and stability. After its purification via centrifugation, microscopy analysis corroborates the format and showed the presence of propolis around silver nanoparticle. X-ray diffraction peaks were attributed to the main planes of the metallic silver crystalline structure; meanwhile infrared spectroscopy demonstrated the main groups responsible for silver reduction, represented by similar to 22% of AgNP-P indicates by thermal analysis. Our product revealed an important antimicrobial activity indicating a synergism between propolis and silver nanoparticles as expected and promising to be an effective antimicrobial product to be used in infections.