Intron sequence of the taurocyamine kinase gene as a marker to investigate genetic variation of Paragonimus species in Japan and the origins of triploidy in P. westermani.
Intron sequence of the taurocyamine kinase gene as a marker to investigate genetic variation of Paragonimus species in Japan and the origins of triploidy in P. westermani.
复制标题
牛磺氰胺激酶基因的内含子序列作为标记来研究日本并殖吸虫物种的遗传变异和韦氏并殖吸虫三倍体的起源。
DOI：
10.1093/trstmh/trv109
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Petney TN.
中科院分区：
文献类型：
--
作者：
Saijuntha W;Tantrawatpan C;Jarilla BR;Agatsuma T;Andrews RH;Petney TN.
背景并殖吸虫病是由并殖吸虫属肺吸虫引起的食源性寄生虫感染。几种并殖吸虫是日本特有的：P.韦斯特曼尼（二倍体和三倍体）P。宫崎，P. ohirai 和 P.伊乐村。这些肺吸虫的分类地位和遗传变异仍知之甚少。方法利用牛磺氰胺激酶基因(TKD1int2)区域1的第二个内含子探讨二倍体和三倍体P的遗传变异和分化。韦斯特马尼，以及P。宫崎，P. ohiraian 和 P. iloktsuenens 源自日本。结果我们发现 P.iloktsuenens 具有高水平的种内变异。韦斯特马尼，但在研究的其他物种中只有低水平的变异。单倍型网络和系统发育树分析证明了P.的姐妹群关系。 ohiraian 和 P. iloktsuenenensis 和 P. 系统发育上的远缘关系。西马尼与其他物种。除三倍体P 之外的所有个体。韦斯特曼尼是纯合子。每个三倍体至少含有一个与千叶县大多数二倍体中所见的等位基因相似的等位基因，以及一个与大分县二倍体中所见的等位基因相似的等位基因。一个三倍体包含三个不同的序列。结论我们的研究结果表明TKD1int2区域是研究并殖吸虫种遗传变异和系统发育的合适标记，并为并殖吸虫三倍体的起源提供线索。韦斯特马尼。
BackgroundParagonimiasis is a foodborne parasitic infection caused by lung flukes of the genusParagonimus. Several species ofParagonimusare endemic in Japan:P. westermani(diploid and triploid)P. miyazakii, P. ohiraiandP. iloktsuenensis. The taxonomic status and genetic variability of these lung flukes remains poorly understood.MethodsThe second intron of domain 1 of the taurocyamine kinase gene (TKD1int2) region was used to explore genetic variation and differentiation of diploid and triploidP. westermani, as well asP. miyazakii,P. ohiraiandP. iloktsuenensisoriginating from Japan.ResultsWe found high levels of intraspecific variation inP. westermani, but only low levels of variation within the other species studied. Haplotype network and phylogenetic tree analyses demonstrated the sister-group relationship ofP. ohiraiandP. iloktsuenensisand the phylogenetically distant relationship ofP. westermaniwith the other species. All individuals except for triploidP. westermaniwere homozygous. Each triploid contained at least one allele similar to that seen in most diploids from Chiba and one allele resembling that seen in diploids from Oita. One triploid contained three different sequences.ConclusionsOur findings suggested that the TKD1int2 region is a suitable marker for use in studying the genetic variation and phylogenetics ofParagonimusspecies, as well as providing clues to the origins of triploidy inP. westermani.