Full-length complementary DNA of hepatitis C virus genome from an infectious blood sample
Full-length complementary DNA of hepatitis C virus genome from an infectious blood sample
复制标题
DOI：
10.1002/hep.510270242
复制
发表时间：
1998-02-01
期刊：
影响因子：
13.5
通讯作者：
Miyamura, T
中科院分区：
文献类型：
--
作者：
Aizaki, H;Aoki, Y;Miyamura, T
从一名丙型肝炎病毒（HCV）携带者的血液样本中构建了全长互补DNA （cDNA）克隆。携带者的血液最终被输给了一名患者，该患者后来在输血后患上了典型的丙型肝炎。这种病毒也被证明对黑猩猩具有传染性。我们总共获得了12个重叠的cDNA片段，覆盖了整个HCV基因组。通过亚克隆和测序，我们选择了被认为构成主要群体的克隆，我们还在基因组的3'端检测到98个碱基对的额外序列，在确定重叠序列后，我们将这些片段组合成全长cDNA。由包膜蛋白高变区核苷酸序列决定，供体中的HCV群体是异质性的，但在传播后，受体中选择了少数病毒克隆。此前，将相同的血液样本注射到黑猩猩体内时，也观察到类似的病毒种群趋同现象。有趣的是，在受体和黑猩猩的急性期分离的病毒克隆在高变区具有与全长cDNA克隆相同的序列。本研究构建的HCV全长cDNA克隆可能来源于感染性病毒克隆。
We constructed a full-length complementary DNA (cDNA) clone of hepatitis C virus (HCV) from a blood sample of an HCV carrier. The blood from the carrier was eventually transfused to a patient who later developed typical posttransfusion hepatitis C. It was also shown to be infectious to chimpanzees. We obtained 12 overlapping cDNA fragments altogether, covering the entire HCV genome. By subcloning and sequencing, clones considered to constitute the major population were selected, We could also detect 98 base pairs of extra sequences at the 3' end of the genome, After confirming the overlapping sequences, we combined the fragments to make a full-length cDNA. The HCV population in the donor was heterogeneous, as determined by their nucleotide sequences of the hypervariable region in envelope protein, but a few virus clones were selected in the recipient after transmission. The similar convergence of the virus population was previously observed when the same blood sample was injected into a chimpanzee. Interestingly, virus clones isolated during the acute phase in the recipient and the chimpanzee had sequences in the hypervariable region identical to that of the full-length cDNA clone. The full-length cDNA clone of HCV constructed in this study may originate from infectious virus clones.