INDUCTION OF PEMPHIGUS IN NEONATAL MICE BY PASSIVE TRANSFER OF IGG FROM PATIENTS WITH THE DISEASE
INDUCTION OF PEMPHIGUS IN NEONATAL MICE BY PASSIVE TRANSFER OF IGG FROM PATIENTS WITH THE DISEASE
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DOI：
10.1056/nejm198205203062001
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发表时间：
1982-01-01
影响因子：
158.5
通讯作者：
DIAZ, LA
中科院分区：
文献类型：
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作者：
ANHALT, GJ;LABIB, RS;DIAZ, LA
通过将来自5名寻常型天疱疮患者的IgG组分以1.5 - 16 mg/g体重/天的剂量被动转移到新生Balb/c小鼠中，检查了循环自身抗体在寻常型天疱疮发病机制中的作用。静脉注射天疱疮患者IgG的55只小鼠中有39只出现具有天疱疮组织学、超微结构和免疫荧光特征的皮肤水疱和糜烂，而给予正常人IgG的58只对照小鼠中无一例出现。具有高滴度天疱疮抗体的IgG组分在诱导疾病方面最有效，并且这种作用是剂量依赖性的。小鼠血清循环IgG滴度与发病程度密切相关（P <0.002）。这项研究强烈支持天疱疮自身抗体在人类寻常天疱疮发病机制中的作用，并证明天疱疮可以被动转移到实验室动物。
The role of circulating autoantibodies in the pathogenesis of pemphigus vulgaris was examined by passively transferring IgG fractions from 5 patients with pemphigus vulgaris into neonatal Balb/c mice, in doses of 1.5-16 mg/g body wt per day. Cutaneous blisters and erosions with the histologic, ultrastructural and immunofluorescence features of pemphigus occurred in 39 of 55 mice given i.v. injections of IgG from patients with pemphigus and in none of 58 control mice given normal human IgG. IgG fractions with high titers of pemphigus antibodies were most effective in inducing disease, and this effect was dose dependent. Titers of circulating IgG in mouse serum closely correlated with the extent of disease induced (P < 0.002). This study strongly supports the proposed role of pemphigus autoantibodies in the pathogenesis of pemphigus vulgaris in human beings and demonstrates that pemphigus can be passively transferred to laboratory animals.