Oct-3/4 repression accelerates differentiation of neural progenitor cells in vitro and in vivo
Oct-3/4 repression accelerates differentiation of neural progenitor cells in vitro and in vivo
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DOI：
10.1016/j.molbrainres.2004.08.021
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发表时间：
2004-12-06
期刊：
影响因子：
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通讯作者：
Okazawa, H
中科院分区：
文献类型：
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作者：
Okuda, T;Tagawa, K;Okazawa, H
Oct-3/4（Oct-3/Oct-4/POU 5 F1）是胚胎干（ES）细胞分化的关键调节因子，尽管其在分化组织中持续存在的组织干细胞中的作用尚未显示。在这里，我们发现Oct-3/4在由神经干细胞和神经祖细胞组成的神经球（NS）中表达，并且通过使用腺病毒载体上调或下调Oct-3/4影响细胞命运。Oct-3/4下调加速祖细胞的神经元分化，而其持续表达阻止神经元分化。将神经球移植到成年大鼠脑中表明，Oct-3/4的下调促进了体内NS细胞的分化。我们的研究结果表明，Oct-3/4是NS细胞分化的重要调节因子，并表明Oct-3/4的调节可能是NS细胞临床应用的有用工具。(C)2004 Elsevier B. V.保留所有权利。
Oct-3/4 (O1ct-3/Oct-4/POU5F1) is a critical regulator of embryonic stem (ES) cell differentiation, though its role in tissue stem cells that persist in differentiated tissues has not been shown. Here, we show that Oct-3/4 is expressed in neurospheres (NS) composed of neural stem cells and neural progenitor cells and that up- ordown-regulation of Oct-3/4 by using adenovirus, vectors influences cell fate. Oct-3/4 downregulation accelerates neuronal differentiation of progenitor cells while its sustained expression prevents neuronal differentiation. Transplantation of neurospheres into the adult rat brain shows that down-regulation of Oct-3/4 promotes differentiation of NS cells in vivo. Our findings indicate that Oct-3/4 is an essential regulator of NS cell differentiation and suggest that the modulation of Oct-3/4 could be a useful tool in clinical application of NS cells. (C) 2004 Elsevier B.V. All rights reserved.