Effect of the serum concentration of the growth medium on the sodium pump site density of cultured HeLa cells.
Effect of the serum concentration of the growth medium on the sodium pump site density of cultured HeLa cells.
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生长培养基的血清浓度对培养的 HeLa 细胞钠泵位点密度的影响。
DOI：
10.1113/expphysiol.1984.sp002799
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发表时间：
1984
影响因子：
--
通讯作者：
J. Lamb
中科院分区：
文献类型：
--
作者：
J. Aiton;J. Lamb
我们测量了培养的HeLa细胞质膜中钠泵位点的密度与细胞生长培养基的血清浓度的关系。在含有血清浓度增加（从1到20% v/v）的培养基中生长，导致钠泵位点数量增加（通过[3H]瓦巴因的特异性结合来测量）和泵活性增加（通过对瓦巴因敏感的86Rb内流来测量）。时间过程研究表明：(1)新的钠泵位点在转移到血清浓度升高的培养基后3-6小时首次出现；(2)蛋白质合成抑制剂环己亚胺和放线菌素d完全消除了血清介导的新钠泵位点的增加。这些结果表明，血清反应的发展需要重新合成蛋白质。对负责启动新钠泵位点合成的血清因子的初步表征表明，该活性与高分子量血清分数（大于50000）有关。血清效应与其他启动新泵位点合成的实验操作（在低钾+培养基中生长，在Li+培养基中生长和外源ATP预处理）之间的不同类型的相互作用表明，在培养的HeLa细胞中，控制钠泵位点合成的途径可能不止一种。
The density of sodium pump sites in the plasma membrane of cultured HeLa cells has been measured as a function of the serum concentration of the cell growth medium. Growth in media containing increased concentrations of serum (from 1 to 20% v/v) leads to an increase in sodium pump site numbers (as measured by the specific binding of [3H]ouabain) and pump activity (as measured by the ouabain-sensitive 86Rb influx). Time-course studies show that (1) new sodium pump sites first appear some 3-6 h after transfer to medium containing an elevated serum concentration and (2) the serum-mediated increase in new sodium pump sites is completely abolished by the protein synthesis inhibitors, cycloheximide and actinomycin D. These results suggest that de novo protein synthesis is required for the development of the serum response. Preliminary characterization of the serum factor responsible for initiating the synthesis of new sodium pump sites indicates that the activity is associated with a high molecular weight serum fraction (greater than 50000). The different types of interaction seen between the serum effect and other experimental manoeuvres which initiate the synthesis of new pump sites (growth in low-K+ medium, growth in Li+ medium and pre-treatment with exogenous ATP) suggest that there may be more than one pathway for the control of sodium pump site synthesis in cultured HeLa cells.