Phosphorylation of linker histones regulates ATP-dependent chromatin remodeling enzymes
Phosphorylation of linker histones regulates ATP-dependent chromatin remodeling enzymes
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DOI：
10.1038/nsb776
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发表时间：
2002-04-01
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影响因子：
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通讯作者：
Peterson, CL
中科院分区：
文献类型：
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作者：
Horn, PJ;Carruthers, LM;Peterson, CL
染色质重塑酶的ATP依赖性家族的成员在转录、发育、DNA修复和细胞周期控制的调节中发挥关键作用。我们发现，重组活动的ySWI/SNF，hSWI/SNF，xMi-2和xACF的复合物几乎废除了纳入连接组蛋白到核小体阵列基板。这种抑制大部分是独立的连接器组蛋白诱导的折叠的阵列。我们还发现Cdc 2/Cyclin B激酶对连接组蛋白的磷酸化可以通过ySWI/SNF挽救重塑。这些结果表明，接头组蛋白发挥全球性的，全基因组的控制重塑活动，暗示一个新的，强制性的连接组蛋白激酶和ATP依赖性重塑酶之间的耦合。
Members of the ATP-dependent family of chromatin remodeling enzymes play key roles in the regulation of transcription, development, DNA repair and cell cycle control. We find that the remodeling activities of the ySWI/SNF, hSWI/SNF, xMi-2 and xACF complexes are nearly abolished by incorporation of linker histones into nucleosomal array substrates. Much of this inhibition is independent of linker histone-induced folding of the arrays. We also find that phosphorylation of the linker histone by Cdc2/Cyclin B kinase can rescue remodeling by ySWI/SNF. These results suggest that linker histones exert a global, genome-wide control over remodeling activities, implicating a new, obligatory coupling between linker histone kinases and ATP-dependent remodeling enzymes.