Visual experience has opposing influences on the quality of stimulus representation in adult primary visual cortex.
Visual experience has opposing influences on the quality of stimulus representation in adult primary visual cortex.
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DOI：
10.7554/elife.80361
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发表时间：
2022-11-02
期刊：
影响因子：
7.7
通讯作者：
Kuhlman SJ
中科院分区：
文献类型：
--
作者：
Jeon BB;Fuchs T;Chase SM;Kuhlman SJ
短暂的黑暗暴露，通常持续7-10天，然后重新引入光线，是一种新兴的治疗方法，用于改善成年后去除遮挡的弱视受试者的视力恢复。黑暗暴露启动稳态机制，与光诱导的细胞信号传导通路的变化一起，导致成年人的幼年样可塑性的重新参与，使得先前被剥夺的输入可以获得皮层领土。黑暗暴露本身可能会降低视觉反应，这可能会限制黑暗暴露治疗的最佳持续时间。为了确定八天的黑暗暴露是否对经典光栅刺激的反应产生持久的负面影响，使用双光子钙成像记录了清醒头部固定小鼠在黑暗暴露之前和之后的神经活动。使用分类器评估的神经辨别力在黑暗暴露后短暂降低;在广泛的空间频率范围内反应可靠性的降低可能导致了这种破坏。可辨别性和可靠性均恢复。固定分类器被用来证明，刺激代表反弹到原来的，剥夺前的状态，因此黑暗暴露似乎并没有对视觉处理产生持久的负面影响。出乎意料的是，我们发现黑暗暴露显着稳定的方向偏好和信号相关性。我们的研究结果表明，自然视觉施加了破坏性的影响，对经典的光栅刺激的刺激偏好的稳定性，并在同一时间，提高了神经辨别力的低和高空间频率的刺激。
Transient dark exposure, typically 7–10 days in duration, followed by light reintroduction is an emerging treatment for improving the restoration of vision in amblyopic subjects whose occlusion is removed in adulthood. Dark exposure initiates homeostatic mechanisms that together with light-induced changes in cellular signaling pathways result in the re-engagement of juvenile-like plasticity in the adult such that previously deprived inputs can gain cortical territory. It is possible that dark exposure itself degrades visual responses, and this could place constraints on the optimal duration of dark exposure treatment. To determine whether eight days of dark exposure has a lasting negative impact on responses to classic grating stimuli, neural activity was recorded before and after dark exposure in awake head-fixed mice using two-photon calcium imaging. Neural discriminability, assessed using classifiers, was transiently reduced following dark exposure; a decrease in response reliability across a broad range of spatial frequencies likely contributed to the disruption. Both discriminability and reliability recovered. Fixed classifiers were used to demonstrate that stimulus representation rebounded to the original, pre-deprivation state, thus dark exposure did not appear to have a lasting negative impact on visual processing. Unexpectedly, we found that dark exposure significantly stabilized orientation preference and signal correlation. Our results reveal that natural vision exerts a disrupting influence on the stability of stimulus preference for classic grating stimuli and, at the same time, improves neural discriminability for both low and high-spatial frequency stimuli.