The effect of continuous intraventricular infusion of L1 and NCAM antibodies on spatial learning in rats
The effect of continuous intraventricular infusion of L1 and NCAM antibodies on spatial learning in rats
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DOI：
10.1016/s0166-4328(96)00046-0
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发表时间：
1996-11-01
影响因子：
2.7
通讯作者：
Welzl, H
中科院分区：
文献类型：
--
作者：
Arami, S;Jucker, M;Welzl, H
最近的研究表明，神经细胞黏附分子L1和NCAM在记忆存储机制中发挥了作用。在本研究中，我们分析了连续脑室注射针对L1(AntiL1)或NCAM(AntiNCAM)的多克隆抗体对雄性Wistar大鼠在空间学习任务(Morris水迷宫)的习得和保持中的作用。在这项任务中，动物们必须学习隐藏在水箱中的逃生平台的空间位置，才能逃到上面。在获得任务的过程中，持续输注抗神经细胞黏附分子的动物--但不输注抗L1的动物--与对照组相比，表现出日依赖的学习减弱(P=0.001)。对照组注射赋形剂(PBS)或抗肝细胞膜多克隆抗体。当在保持试验(转移试验)中移除逃生平台时，持续注射抗L1和持续注射抗神经细胞黏附分子的动物与对照动物相比，表现出搜索模式受损(分别为P=0.001.0 5和0.0 4)。而对照组动物在正确象限寻找平台的时间高达46%，而注射antiL1和antiNCAM的动物在这个象限花费的时间更接近随机水平(分别为30.5%和36.5%)。目前的数据为两个黏附分子L1和NCAM参与突触可塑性的记忆存储提供了额外的支持。
Recent studies suggest a role of the neural cell adhesion molecules L1 and NCAM in mechanisms of memory storage. In the present study we analyzed the effect of continuous intraventricular infusion of polyclonal antibodies directed against L1 (antiL1) or NCAM (antiNCAM) on the performance of male Wistar rats during the acquisition and retention of a spatial learning task (Morris water-maze). In this task animals have to learn the spatial position of a hidden escape platform in a water tank to escape onto it. During acquisition of the task animals with continuous infusion of antiNCAM - but not those infused with antiL1- showed day-dependent attenuated learning in comparison to controls (P=0.001). Control animals were either injected with vehicle (PBS) or with polyclonal antibodies raised against liver cell membrane. When the escape platform was removed during the retention test (transfer test), the performance of animals continuously infused with antiL1 as well as those continuously infused with antiNCAM showed an impaired search pattern when compared with the performance of control animals (P=0.001 and 0.04, respectively). Whereas control animals spent up to 46% of their time searching for the platform in the correct quadrant, the time antiL1- and antiNCAM-infused animals spent in this quadrant was closer to chance level (30.5% and 36.5%, respectively). The present data provide additional support for an involvement of the two adhesion molecules L1 and NCAM in synaptic plasticity underlying memory storage.