Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran
Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran
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DOI：
10.1126/science.284.5418.1359
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发表时间：
1999-05-21
期刊：
影响因子：
56.9
通讯作者：
Zheng, YX
中科院分区：
文献类型：
--
作者：
Wilde, A;Zheng, YX
Ran是一种小的鸟苷三磷酸酶，除了其在核运输中的作用外，还具有其他功能，鸟苷三磷酸结合的Ran，而不是鸟苷二磷酸结合的Ran，刺激了非洲爪蟾精子上中心体的星形微管的聚合。此外，效应结构域突变的Ran等位基因（RanL 43 E）诱导了微管星形细胞的形成和纺锤体的组装，在没有精子核的情况下，以γ TuRC（γ-微管蛋白环复合物）和XMAP 215（非洲爪蟾微管相关蛋白）依赖的方式。因此，Ran可能是有丝分裂过程中调控微管聚合的关键信号分子。
Ran, a small guanosine triphosphatase, is suggested to have additional functions beyond its well-characterized role in nuclear trafficking, Guanosine triphosphate-bound Ran, but not guanosine biphosphate-bound Ran, stimulated polymerization of astral microtubules from centrosomes assembled on Xenopus sperm, Moreover, a Ran allele with a mutation in the effector domain (RanL43E) induced the formation of microtubule asters and spindle assembly, in the absence of sperm nuclei, in a gamma TuRC (gamma-tubulin ring complex)- and XMAP215 (Xenopus microtubule associated protein)-dependent manner. Therefore, Ran could be a key signaling molecule regulating microtubule polymerization during mitosis.