PASSIVE ELECTRICAL PROPERTIES OF MICROORGANISMS .3. CONDUCTIVITY OF ISOLATED BACTERIAL CELL WALLS
PASSIVE ELECTRICAL PROPERTIES OF MICROORGANISMS .3. CONDUCTIVITY OF ISOLATED BACTERIAL CELL WALLS
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DOI：
10.1016/s0006-3495(68)86506-3
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发表时间：
1968-01-01
影响因子：
3.4
通讯作者：
MARQUIS, RE
中科院分区：
文献类型：
--
作者：
CARSTENSEN, EL;MARQUIS, RE
对分离的溶血微球菌细胞壁的介电特性进行了研究，以更坚定地确立壁相关离子在完整细菌细胞的低频电流传导中起主要作用的观点。离体壁的电导率约为0.40mho/m，如果离体壁中与固定电离基团有关的反离子的平均迁移率等于自由溶液中的Na离子的平均迁移率，则考虑到测量的电导率所需的固定电荷浓度在湿壁体积的75~95meq/1之间。对壁面聚合物中可滴定的氨基和羧基数量的估计表明，电导率与净壁电荷的关系比与总壁电荷的关系更密切。测量的壁电导率被用来预测全细胞电导率的值为0.15[正负]0.03mho/m。这一预测接近0.25[正负]0.05mho/m的实测值。人们认为，值的差异很大程度上与隔离过程中墙结构和成分的变化有关。
The dielectric properties of isolated Micrococcus lysodeikticus cell walls were studied to establish more firmly the view that wall-associated ions play a major role in the conduction of low frequency electric current by intact bacterial cells. The conductivity of isolated walls was found to be about 0.40 mho/m. If counterions associated with fixed ionized groups in the wall have average mobilities equal to that of Na ions in free solution, the fixed charge concentration required to account for the measured conductivity is between 75 and 95 meq/1 of wet wall volume. Estimates of the numbers of titratable amino and carboxyl groups in wall polymers indicate that conductivity is more closely related to net wall charge than to total wall charge. The measured wall conductivity was used to predict a value of 0.15 [plus or minus] 0.03 mho/m for whole cell conductivity. This prediction is close to the measured value of 0.25 [plus or minus] 0.05 mho/m it is thought that much of the disparity in values is related to changes in wall structure and composition during the isolation procedures.