Keloid skin scars: the influence of hyperbaric oxygenation on fibroblast growth and on the expression of messenger RNA for insulin like growth factor and for transforming growth factor
Keloid skin scars: the influence of hyperbaric oxygenation on fibroblast growth and on the expression of messenger RNA for insulin like growth factor and for transforming growth factor
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DOI：
10.1007/s11626-011-9418-3
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发表时间：
2011-08-01
影响因子：
2.1
通讯作者：
Ortiz-Monasterio, Fernando
中科院分区：
文献类型：
--
作者：
Romero-Valdovinos, Mirza;Cardenas-Mejia, Alexander;Ortiz-Monasterio, Fernando
伤口愈合可导致含有非典型成纤维细胞和过量细胞外基质成分的瘢痕疙瘩的形成。高压氧（HBO）是指在升高的大气压力下暴露于纯氧，被认为是治疗有问题的伤口的一种有价值的补充方法。通过半定量 RT-PCR 在从同一患者的瘢痕疙瘩和非伤口相关皮肤成纤维细胞中获得的成纤维细胞中测定 HBO 对 1 型胰岛素样生长因子 (ILGF-1) 和转化生长因子 β (TGF-β) 信使 RNA 表达的影响。 ILGF-1 和 TGF-β 是伤口再生过程中主要的有丝分裂原。我们发现，与正常氧分压相比，慢性暴露于高压氧后，成纤维细胞的生长以及瘢痕疙瘩和非瘢痕疙瘩成纤维细胞中 ILGF-1 和 TGF-β 信使的表达减少。
Wound healing can result in the development of keloid scars that contain atypical fibroblasts and an overabundance of extracellular matrix components. Hyperbaric oxygenation (HBO) refers to exposure to pure oxygen under increased atmospheric pressure and is recognized as a valuable supplementary method of treatment for problematic wounds. The effect of HBO in the expression of insulin-like growth factor type 1 (ILGF-1) and transforming growth factor beta (TGF-beta) messenger RNAs was determined by semiquantitative RT-PCR in fibroblasts obtained from keloid scars and nonwound involved skin fibroblast from the same patient. ILGF-1 and TGF-beta are the principal mitogens during wound regeneration. We found a decrease in the growth of fibroblasts and in the expression of ILGF-1 and TGF-beta messengers in keloid and nonkeloid fibroblast after chronic exposition to hyperbaric oxygenation compared with normal oxygen partial pressure.