Nano- and Microspheres Containing Inorganic and Biological Nanoparticles: Self-Assembly and Electron Tomographic Analysis
Nano- and Microspheres Containing Inorganic and Biological Nanoparticles: Self-Assembly and Electron Tomographic Analysis
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含有无机和生物纳米颗粒的纳米球和微球：自组装和电子断层扫描分析
DOI：
10.1021/jacs.0c11944
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发表时间：
2021
影响因子：
15
通讯作者：
Nakamura Eiichi
中科院分区：
文献类型：
--
作者：
Sekine Ryosuke;Ravat Prince;Yanagisawa Haruaki;Liu Chao;Kikkawa Masahide;Harano Koji;Nakamura Eiichi
有机富勒烯两亲物在水中表现出不同的行为，形成囊泡，胶束，LB膜，和各向异性的纳米结构。我们发现，（4-庚基苯基）5C 60-K+的有机溶液通过水或缓冲液逐渐在原位质子化产生相应的质子化分子，（4-庚基苯基）5C 60 H，其自组装形成直径从30 nm到2.5 μm的均匀的有机富勒烯纳米球和微球（富勒球），其由缓冲液的制备或pH控制。通过使用有机染料，无机纳米颗粒，蛋白质和病毒的水溶液，我们将这些实体封装在富勒烯中。这种通过自组装的方法不同于通常需要聚合以构建坚固壳的有机核-壳颗粒的其他制备。该球体是完全无定形的，在真空下热稳定性高达300 °C，并且耐电子辐照，我们发现了该球体用于纳米颗粒和生物材料的电子断层成像的非常规用途。
Organofullerene amphiphiles show diverse behaviors in water, forming vesicles, micelles, Langmuir–Blodgett films, and anisotropic nanostructures. We found that gradual in situ protonation of an organic solution of (4-heptylphenyl)5C60–K+by water or buffer generates the corresponding protonated molecule, (4-heptylphenyl)5C60H, which self-assembles to form nano- and microspheres of organofullerene (fullerspheres) with uniform diameters ranging from 30 nm to 2.5 μm that are controlled by the preparation or pH of the buffer. By using an aqueous solution of an organic dye, inorganic nanoparticle, protein, and virus, we encapsulated these entities in the fullersphere. This approach via self-assembly is distinct from other preparations of organic core–shell particles that generally require polymerization for the construction of a robust shell. The sphere is entirely amorphous, thermally stable up to 300 °C under vacuum, and resistant to electron irradiation, and we found the unconventional utility of the sphere for electron tomographic imaging of nanoparticles and biomaterials.