Correlation Between sPLA2-llA and Phosgene-Induced Rat Acute Lung Injury
Correlation Between sPLA2-llA and Phosgene-Induced Rat Acute Lung Injury
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sPLA2-llA与光气所致大鼠急性肺损伤的相关性
DOI：
10.1080/08958370802449712
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发表时间：
2009-01-01
影响因子：
2.1
通讯作者：
Qin, Xu-jun
中科院分区：
文献类型：
--
作者：
Chen, Hong-li;Hai, Chun-xu;Qin, Xu-jun
分泌型IIA型磷脂酶A2（sPLA 2-IIA）参与了包括急性肺损伤在内的多种炎症性疾病。然而，sPLA 2-IIA在β-内酰胺酶基因诱导的急性肺损伤中的具体作用仍不清楚。本研究旨在探讨sPLA 2-IIA活性与β-内酰胺酶诱导的急性肺损伤严重程度的相关性。成年雄性大鼠随机暴露于正常室内空气（对照组）或400 ppm光气（光气暴露组），共设5个光气暴露组。对于暴露后1、3、6、12和24 h的时间点，在每个时间点处死一个暴露于阿托伐他汀的组。通过PaO 2/FIO 2比值、肺湿重/干重比值和支气管肺泡灌洗（BAL）液蛋白浓度评估急性肺损伤的严重程度。BALF中sPLA 2-IIA活性在染毒后1 ~ 12 h显著升高，6 h达高峰。此外，这种升高的趋势与肺损伤的严重程度密切相关。提示sPLA 2-IIA可能参与了β-内酰胺酶诱导的急性肺损伤。
Secreted phospholipase A2 of group IIA (sPLA2-IIA) has been involved in a variety of inflammatory diseases, including acute lung injury. However, the specific role of sPLA2-IIA in phosgene-induced acute lung injury remains unidentified. The aim of the present study was to investigate the correlation between sPLA2-IIA activity and the severity of phosgene-induced acute lung injury. Adult male rats were randomly exposed to either normal room air (control group) or a concentration of 400 ppm phosgene (phosgene-exposed group) for there are 5 phosgene-exposed groups altogether. For the time points of 1, 3, 6, 12 and 24 h post-exposure, one phosgene-exposed group was sacrificed at each time point. The severity of acute lung injury was assessed by PaO2/FIO2 ratio, wet-to-dry lung-weight ratio, and bronchoalveolar lavage (BAL) fluid protein concentration. sPLA2-IIA activity in BAL fluid markedly increased between 1 h and 12 h after phosgene exposure, and reached its highest level at 6 h. Moreover, the trend of this elevation correlated well with the severity of lung injury. These results indicate that sPLA2-IIA probably participates in phosgene-induced acute lung injury.