Altered sirtuin deacetylase gene expression in patients with a mood disorder
Altered sirtuin deacetylase gene expression in patients with a mood disorder
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情绪障碍患者中沉默调节蛋白脱乙酰酶基因表达的改变
DOI：
10.1016/j.jpsychires.2011.01.016
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发表时间：
2011-08-01
影响因子：
4.8
通讯作者：
Watanabe, Yoshifumi
中科院分区：
文献类型：
--
作者：
Abe, Naoko;Uchida, Shusaku;Watanabe, Yoshifumi
Sirtuins 是 NAD+ 依赖性酶家族，通过各种蛋白质的脱乙酰化来调节细胞功能。尽管最近的报告表明脱乙酰酶（即组蛋白脱乙酰酶）在情绪障碍和抗抑郁作用中发挥重要作用，但去乙酰化酶在情绪障碍的病理生理学中的参与很大程度上尚不清楚。在这项研究中，我们旨在确定情绪障碍患者外周白细胞中沉默调节蛋白 mRNA 表达是否存在变化。此外，为了检查改变的沉默调节蛋白 mRNA 表达是否具有状态依赖性或特质依赖性，对处于缓解状态的情绪障碍患者进行了评估。我们使用定量实时聚合酶链反应来测量重度抑郁症 (MDD) 或双相情感障碍 (BPD) 患者在抑郁和缓解状态以及正常健康受试者外周血白细胞中七种去乙酰化酶亚型 (SIRT1-7) 的 mRNA 水平。与健康对照相比，处于抑郁状态的MDD和BPD患者中SIRT1、2和6 mRNA水平显着降低，而处于缓解状态的MDD和BPD患者中这些mRNA的表达与健康对照相当。因此，我们的数据表明 SIRT1、2 和 6 表达的改变是状态依赖性的，并且可能与情绪障碍的发病机制和/或病理生理学相关。 (C) 2011 Elsevier Ltd. 保留所有权利。
Sirtuins are a family of NAD+-dependent enzymes that regulate cellular functions through deacetylation of various proteins. Although recent reports have suggested an important role of deacetylases (i.e., histone deacetylases) in mood disorders and antidepressant action, the involvement of sirtuins in the pathophysiology of mood disorders is largely unknown. In this study, we aimed to determine whether there are alterations in sirtuin mRNA expression in peripheral white blood cells of patients with a mood disorder. Also, to examine whether the altered sirtuin mRNA expression is state- or trait-dependent, mood disorder patients who were in a remissive state were assessed. We used quantitative real-time polymerase chain reaction to measure the mRNA levels of seven sirtuin isoforms (SIRT1-7) in peripheral white blood cells of patients with major depressive disorder (MDD) or bipolar disorder (BPD) during depressive and remissive states and in normal healthy subjects. The SIRT1, 2 and 6 mRNA levels in MDD and BPD patients decreased significantly in those who were in a depressive state compared to healthy controls, whereas the expression of those mRNAs in both MDD and BPD of patients in a remissive state were comparable to those in healthy controls. Thus, our data suggest that altered SIRT1, 2 and 6 expression is state-dependent and might be associated with the pathogenesis and/or pathophysiology of mood disorders. (C) 2011 Elsevier Ltd. All rights reserved.