Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor.
Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor.
复制标题
卵巢基因表达在没有图形的情况下，卵母细胞特异性转录因子。
DOI：
10.1186/1471-213x-7-67
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发表时间：
2007-06-13
影响因子：
--
通讯作者：
Dean, Jurrien
中科院分区：
文献类型：
--
作者：
Joshi, Saurabh;Davies, Holly;Sims, Lauren Porter;Levy, Shawn E.;Dean, Jurrien
哺乳动物卵巢卵泡发生是一个涉及生殖细胞和体细胞相互作用的复杂过程。转录因子、细胞粘附分子和生长因子的精心表达是成功的必要条件。我们已经确定了一种生殖细胞特异性的基本螺旋-环-螺旋转录因子，FIGLA (factor In the GermLine, Alpha)，并证明其参与两个独立的发育过程：原始卵泡的形成和透明带基因的协调表达。利用Figla空白小鼠系，我们使用微阵列和基因表达序列分析（SAGE）相结合的方法来鉴定潜在的下游靶基因。通过高严格的切断，我们发现FIGLA是协调NALP基因家族、已知卵母细胞特异性表达基因和一组功能未注释基因表达的关键调控分子。FIGLA还抑制男性生殖细胞特异性基因的表达，否则可能会破坏正常的卵子发生。这些数据表明FIGLA是卵母细胞特异性基因的中心调节因子，在卵泡发生、受精和早期发育中发挥作用。
Ovarian folliculogenesis in mammals is a complex process involving interactions between germ and somatic cells. Carefully orchestrated expression of transcription factors, cell adhesion molecules and growth factors are required for success. We have identified a germ-cell specific, basic helix-loop-helix transcription factor, FIGLA (Factor In the GermLine, Alpha) and demonstrated its involvement in two independent developmental processes: formation of the primordial follicle and coordinate expression of zona pellucida genes. Taking advantage of Figla null mouse lines, we have used a combined approach of microarray and Serial Analysis of Gene Expression (SAGE) to identify potential downstream target genes. Using high stringent cutoffs, we find that FIGLA functions as a key regulatory molecule in coordinating expression of the NALP family of genes, genes of known oocyte-specific expression and a set of functionally un-annotated genes. FIGLA also inhibits expression of male germ cell specific genes that might otherwise disrupt normal oogenesis. These data implicate FIGLA as a central regulator of oocyte-specific genes that play roles in folliculogenesis, fertilization and early development.