Microspectroscopy of single proliferating HeLa cells
Microspectroscopy of single proliferating HeLa cells
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DOI：
10.1016/j.vibspec.2005.02.028
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发表时间：
2005-07-29
影响因子：
2.5
通讯作者：
Diem, M
中科院分区：
文献类型：
--
作者：
Boydston-White, S;Chernenko, T;Diem, M
迄今为止观察到的正常和异常组织之间的光谱差异似乎是由于根据细胞的生物化学不同的光谱模式的不同平均过程，由于其成熟，分化和发育的状态。因此，疾病扰乱了细胞在成熟、分化和发育的不同阶段的分布。以前对正常与肿瘤细胞和组织的FTIR显微光谱研究已经证明了吸收强度和带形的差异，特别是在低频（1200-1000 cm（-1））光谱区域。在这项研究中，我们进一步研究了由于细胞增殖而发生的剧烈生化和形态变化所导致的光谱变化。(c)2004 Elsevier B. V.保留所有权利。
Spectral differences between normal and abnormal tissue observed to date appear to be due to different averaging processes of spectral patterns that differ according to the cell's biochemistry, due to its state of maturation, differentiation, and development. Thus, disease perturbs the distribution of cells in the different stages of maturation, differentiation, and development. Previous FTIR microspectroscopic studies of normal versus neoplastic cells and tissues have demonstrated differences in the absorption intensities and band-shapes, particularly in the low frequency (1200-1000 cm(-1)) spectral region. In this study, we further investigated the spectral changes due to the drastic biochemical and morphological changes occurring as a consequence of cell proliferation. (c) 2004 Elsevier B.V. All rights reserved.