CIRCULATING IMMUNE-COMPLEXES AND INVITRO-CELL REACTIVITY IN PARACOCCIDIOIDOMYCOSIS
CIRCULATING IMMUNE-COMPLEXES AND INVITRO-CELL REACTIVITY IN PARACOCCIDIOIDOMYCOSIS
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DOI：
10.1007/bf01837195
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发表时间：
1982-01-01
期刊：
影响因子：
5.5
通讯作者：
YARZABAL, L
中科院分区：
文献类型：
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作者：
ARANGO, M;OROPEZA, F;YARZABAL, L
采用人淋巴母细胞Raji细胞放射免疫测定法（Raji）和125i标记补体成分C1q结合测定法（C1q- ba）测定14例活动性或非活动性副球孢子菌病（Pcm）患者和15名健康对照者血清中的循环免疫复合物（CIC）。C1q-BA显示，除1例患者外，其余患者血清中CIC水平均显著升高。Raji检测结果显示，8名活跃患者中有4名（62%）和6名不活跃患者中有2名（33%）的CIC水平明显高于对照组。通过Raji试验，仅在活动期患者中检测到CIC水平显著升高。对1例全身性感染淋巴细胞反应性低下患者进行血清检测，发现血清中含有抑制异体淋巴细胞和正常淋巴细胞体外增殖的因子。刺激前对患者淋巴细胞进行预培养可恢复其增殖能力，培养上清中可检测到免疫复合物（IC）。随后将患者血清添加到这种预培养细胞中，不会再引起抑郁。因此，在Pcm中观察到的T细胞反应的抑制是由于IC的存在，IC可能与应答细胞可逆地相互作用和/或激活抑制细胞群，其活性因预培养而降低。
Circulating immune complexes (CIC) were measured by the human lymphoblastoid Raji cell radioimmunoassay (Raji) and by the 125I-labeled complement component C1q binding assay (C1q-BA) in sera from 14 patients with active or inactive forms of paracoccidioidomycosis (Pcm) and from 15 healthy controls. The C1q-BA revealed significantly elevated levels of CIC in the sera of all but 1 of the patients. Four of the 8 active (62%) and 2 of the 6 inactive (33%) patients had CIC levels significantly higher than the controls as determined by the Raji test. Significantly increased levels of CIC were detected only in the active patients by the Raji test. The serum of 1 patient with generalized infection and depressed lymphocyte responsiveness was examined and found to contain a factor which depressed the in vitro proliferation of homologous and normal lymphocytes. Pre-culture of the patients'' lymphocytes before stimulation restored their proliferative capacity and immune complexes (IC) were detectable in the culture supernatants. Subsequent addition of the patients'' serum to such precultured cells did not reinduce the depression. Therefore, the depression of T cell responses observed in Pcm is due to the presence of IC which may interact reversibly with the responding cells and/or activate a suppressor cell population whose activity is diminished by preculture.