Luminescent nanobeads for optical sensing and imaging of dissolved oxygen
Luminescent nanobeads for optical sensing and imaging of dissolved oxygen
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DOI：
10.1007/s00604-008-0047-9
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发表时间：
2009-01-01
影响因子：
5.7
通讯作者：
Klimant, Ingo
中科院分区：
文献类型：
--
作者：
Borisov, Sergey M.;Klimant, Ingo
通过对聚（苯乙烯-嵌段-乙烯基吡咯烷酮）纳米珠与其发光被氧猝灭的金属配体络合物的可寻址染色，制备了各种发光氧纳米传感器。它们在响应0至100%空气饱和度的溶解氧浓度时显示出最佳的灵敏度。基于环化铱（III）与香豆素的配合物的纳米珠由于优异的亮度而特别有前途。纳米传感器几乎在真实的时间内对氧气浓度的变化做出反应，并且能够记录氧气分压的非常快速的变化。通过在频域和时域中测定发光寿命以及通过发光强度的比率测量来获得信号。纳米传感器已被应用于溶解氧的传感和成像，以监测葡萄糖酶促氧化过程中的氧消耗，并监测E.杆菌
A variety of luminescent oxygen nanosensors were prepared by addressable staining of poly(styrene-block-vinylpyrrolidone) nanobeads with metal-ligand complexes whose luminescence is quenched by oxygen. They display optimal sensitivity in responding to dissolved oxygen in concentrations from 0 to 100% air saturation. The nanobeads based on cyclometallated iridium(III) complexes with coumarins are especially promising due to excellent brightnesses. The nanosensors respond virtually in real time to altering oxygen concentration and are capable of recording even very rapid changes in oxygen partial pressure. Signals are obtained by determination of luminescence lifetime in the frequency domain and in the time domain, and by ratiometric measurement of luminescence intensity. The nanosensors have been applied to sensing and imaging of dissolved oxygen, to monitor the consumption of oxygen during enzymatic oxidation of glucose, and to monitoring dissolved oxygen in a growing culture of E. coli.