Resveratrol Reduces the Proinflammatory Effects and Lipopolysaccharide- Induced Expression of HMGB1 and TLR4 in RAW264.7 Cells
Resveratrol Reduces the Proinflammatory Effects and Lipopolysaccharide- Induced Expression of HMGB1 and TLR4 in RAW264.7 Cells
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白藜芦醇减少 RAW264.7 细胞中的促炎作用和脂多糖诱导的 HMGB1 和 TLR4 表达
DOI：
10.1159/000358696
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发表时间：
2014-01-01
影响因子：
--
通讯作者：
Chen, Zhi
中科院分区：
文献类型：
--
作者：
Yang, Ying;Li, Shuping;Chen, Zhi
背景：白藜芦醇（Resveratrol，Res）是一种多酚类抗炎药。我们研究了Res的抗炎作用与高迁移率族蛋白1（HMGB 1）信号通路之间的联系。研究方法：小鼠巨噬细胞样RAW264.7细胞（RAW264.7细胞）未经处理（对照）或用Res、LPS或LPS + Res处理。通过qRT-PCR、western blot和免疫荧光法检测HMGB 1的表达。同时检测TLR 4蛋白和mRNA表达水平。结果：Res能显著降低LPS诱导的RAW264.7细胞IL-6、NO和TNF-α水平。LPS + Res处理的细胞中HMGB 1（mRNA和蛋白）和TLR 4的表达水平低于单独用LPS处理的细胞。结论：Res能阻断LPS诱导的RAW264.7细胞炎症反应。下调HMGB表达可能是Res的作用机制之一，Res还可能影响HMGB 1-TLR 4信号通路中TLR 4的表达。
Background: Resveratrol (Res) is a polyphenol anti-inflammatory agent. We have studied the link between the anti-inflammatory effects of Res and the high mobility group box 1(HMGB1) signaling pathway. Methods: Murine macrophage-like RAW264.7 cells (RAW264.7 cells) were either untreated (control) or treated with Res, LPS, or LPS + Res. Levels of IL-6, NO, and TNF-α were measured by ELISA and colorimetric assays. Expression of HMGB1 was detected by qRT-PCR, western blot, and immunofluorescence assays. Protein and mRNA expression levels of TLR4 were also examined. Results: Res significantly reduced the levels of IL-6, NO, and TNF-α in RAW264.7 cells exposed to LPS. Expression levels of HMGB1 (mRNA and protein) and of TLR4 in the LPS + Res-treated cells were lower than in cells treated with LPS alone. Conclusions: Res can block the inflammatory effects induced by LPS in RAW264.7 cells. Down-regulation of HMGB expression may be one of the mechanisms of action of Res. Res may also influence TLR4 expression in the HMGB1-TLR4 signaling pathway.