In vivo validation of a miniaturized electrochemical oxygen sensor for measuring intestinal oxygen tension
In vivo validation of a miniaturized electrochemical oxygen sensor for measuring intestinal oxygen tension
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DOI：
10.1152/ajpgi.00050.2019
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发表时间：
2019-08-01
影响因子：
4.5
通讯作者：
Potter, Mark A.
中科院分区：
文献类型：
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作者：
Gray, Mark E.;Marland, Jamie R. K.;Potter, Mark A.
电子和微加工技术领域的最新进展导致了用于精密医学领域的植入式医疗设备的发展。通过可植入传感器监测内脏表面组织O-2张力（P-TO2）在许多临床情况下都有潜在的用途，包括肠切除术和吻合患者的围手术期管理。这个概念可以提供一种方法，通过这种方法，治疗可以针对个别患者。本研究描述了一种新型小型化电化学O-2传感器的体内验证，该传感器可提供肠道P-TO2的实时数据。在暴露于缺血（肠系膜上动脉闭塞）和低氧血症（激发分数O-2浓度改变）损伤的麻醉大鼠的小肠浆膜表面放置单个O-2传感器。控制实验表明，传感器可以在体内环境中正常工作并保持稳定。肠系膜上动脉闭塞后肠道P-TO2降低，激发O-2浓度降低。这些结果在恢复血流或通过增加激发的O-2浓度后是可逆的。我们已经成功地开发了一个麻醉大鼠肠道缺血和缺氧模型，用于验证小型化O-2传感器，以提供肠道P-TO2的实时测量。我们的研究结果支持在生理条件下使用大型动物模型进一步验证传感器，为其在监测内脏表面组织O-2张力的临床应用提供证据。新的和值得注意的是，这是使用微型制造的O-2传感器实时连续测量肠道氧张力的第一份报告。通过开发的啮齿动物模型，我们已经验证了该传感器准确测量通过缺血和低氧血症损伤发生的肠道氧合动态和可逆变化的能力。持续监测肠道局部氧合对肠道手术患者的术后监测具有一定的价值。
Recent advances in the fields of electronics and microfabrication techniques have led to the development of implantable medical devices for use within the field of precision medicine. Monitoring visceral surface tissue O-2 tension (P-TO2) by means of an implantable sensor is potentially useful in many clinical situations, including the perioperative management of patients undergoing intestinal resection and anastomosis. This concept could provide a means by which treatment could be tailored to individual patients. This study describes the in vivo validation of a novel, miniaturized electrochemical O-2 sensor to provide real-time data on intestinal P-TO2. A single O-2 sensor was placed onto the serosal surface of the small intestine of anesthetized rats that were exposed to ischemic (superior mesenteric artery occlusion) and hypoxemic (alterations in inspired fractional O-2 concentrations) insults. Control experiments demonstrated that the sensors can function and remain stable in an in vivo environment. Intestinal P-TO2 decreased following superior mesenteric artery occlusion and with reductions in inspired O-2 concentrations. These results were reversible after reinstating blood flow or by increasing inspired O-2 concentrations. We have successfully developed an anesthetized rat intestinal ischemic and hypoxic model for validation of a miniaturized O-2 sensor to provide real-time measurement of intestinal P-TO2. Our results support further validation of the sensors in physiological conditions using a large animal model to provide evidence of their use in clinical applications where monitoring visceral surface tissue O-2 tension is important.NEW & NOTEWORTHY This is the first report of real-time continuous measurements of intestinal oxygen tension made using a microfabricated O-2 sensor. Using a developed rodent model, we have validated this sensor's ability to accurately measure dynamic and reversible changes in intestinal oxygenation that occur through ischemic and hypoxemic insults. Continuous monitoring of local intestinal oxygenation could have value in the postoperative monitoring of patients having undergone intestinal surgery.