Multiscale dynamics in nucleocytoplasmic transport
Multiscale dynamics in nucleocytoplasmic transport
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DOI：
10.1016/j.ceb.2011.11.011
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发表时间：
2012-02-01
影响因子：
7.5
通讯作者：
Singer, Robert H.
中科院分区：
文献类型：
--
作者：
Grunwald, David;Singer, Robert H.
核孔复合体（NPC）一直被认为是细胞核和细胞质之间的点状进出通道。新的数据支持一种不同的观点，即复杂显示出不同的空间动态，其持续时间从毫秒到跨越整个细胞周期的事件不等。中央通道外的离散相互作用位点变得明显，这些位点的运输调节似乎比目前认为的更重要。核孔成分在NPC外高度活跃或影响货物运输远离核孔的命运。NPC是一个高度动态、拥挤的环境——不断装载货物，同时提供基于未折叠蛋白质的选择性。综上所述，这构成了我们如何看待输入/输出动态的新范式，并强调了npc介导的细胞运输的多尺度性质。
The nuclear pore complex (NPC) has long been viewed as a point-like entry and exit channel between the nucleus and the cytoplasm. New data support a different view whereby the complex displays distinct spatial dynamics of variable duration ranging from milliseconds to events spanning the entire cell cycle. Discrete interaction sites outside the central channel become apparent, and transport regulation at these sites seems to be of greater importance than currently thought. Nuclear pore components are highly active outside the NPC or impact the fate of cargo transport away from the nuclear pore. The NPC is a highly dynamic, crowded environment - constantly loaded with cargo while providing selectivity based on unfolded proteins. Taken together, this comprises a new paradigm in how we view import/export dynamics and emphasizes the multiscale nature of NPC-mediated cellular transport.