In vitro anticancer screening of South African plants
In vitro anticancer screening of South African plants
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DOI：
10.1016/j.jep.2008.07.005
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发表时间：
2008-10-28
影响因子：
5.4
通讯作者：
Senabe, J.
中科院分区：
文献类型：
--
作者：
Fouche, G.;Cragg, G. M.;Senabe, J.
研究目的：本研究的目的是评价南非植物的抗癌活性。材料和方法：在南非各地收集植物物种，凭证标本保存在南非国家生物多样性研究所并进行鉴定。制备了植物提取物，并在CSIR上筛选了对三种人类细胞系(乳腺MCF7、肾脏TK10和黑色素瘤LIACC62)的体外抗癌活性。结果：1999-2006年间，共有7500株植物提取物对乳腺MCF7、肾TK10和黑色素瘤UACC62细胞具有体外抗癌活性。根据HITS对细胞株的完全生长抑制作用，将其分为四类。NCI对60个人类癌细胞株进行筛选后，显示中等活性的提取物的命中率为5.9%。对已发表的抗癌特性信息有限的植物的提取物进行了生物测定指导的分离，并分离和鉴定了有效成分。本研究的植物标本数量最多的是菊科植物，富含倍半萜内酯。结论：虽然这些植物的提取物是随机选择的，但其中68%的植物被报道具有药用价值。根据我们的数据，来源植物的无关药用似乎可以作为选择抗癌筛选植物的初步指南。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
Aim of the study: The purpose of the present study is to evaluate South African plants for their anticancer activity.Materials and methods: Plant species were collected throughout South Africa and voucher specimens were deposited and identified at the South African National Biodiversity Institute. Plant extracts were prepared and screened for in vitro anticancer activity against a panel of three human cell lines (breast MCF7, renal TK10 and melanoma LIACC62) at the CSIR. Plant extracts that exhibited anticancer activity against these three human cell lines were screened by the NO against sixty human cancer cell lines organized into sub-panels representing leukaemia, melanoma, cancer of the lung, colon, kidney, ovary, central nervous system, breast and prostate.Results: A total of 7500 plant extracts were screened for in vitro anticancer activity against breast MCF7, renal TK10 and melanoma UACC62 human cell lines between the period 1999 and 2006. Hits were classified into four categories based on their total growth inhibition of the cell lines. A hit rate of 5.9% was obtained for extracts which showed moderate activity and these were screened by the NCI against a panel of sixty human cancer cell lines. The extracts of plant species with limited published information for their anticancer properties were subjected to bioassay-guided fractionation and the active constituents isolated and identified. The largest number of plant specimens in this study was from the family Asteraceae, which is rich in sesquiterpene lactones.Conclusions: Although the extracts of the plants were randomly selected, 68% of these plant species which were hits in the screening programme are reported to be used medicinally. Based on our data, it appears that unrelated medicinal use of the source plants may serve as an initial guide to selection of plants for anticancer screening. (C) 2008 Elsevier Ireland Ltd. All rights reserved.