Multiple spatiotemporal modes of actin reorganization by NMDA receptors and voltage-gated Ca2+ channels
Multiple spatiotemporal modes of actin reorganization by NMDA receptors and voltage-gated Ca2+ channels
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DOI：
10.1073/pnas.212148999
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发表时间：
2002-10-29
影响因子：
11.1
通讯作者：
Narumiya, S
中科院分区：
文献类型：
--
作者：
Furuyashiki, T;Arakawa, Y;Narumiya, S
细胞骨架被认为有助于神经元可塑性的活性依赖过程，如细胞形态的调节和信号蛋白的定位。然而，神经元活动如何控制肌动蛋白细胞骨架仍不清楚。利用海马神经元原代培养中增强的GFP-肌动蛋白的共聚焦成像，我们发现突触活动诱导了多种类型的肌动蛋白重组，包括在脊椎和躯体周围。N-甲基-D-天冬氨酸受体的激活，伴随着局部[Ca~(2+)](I)的升高，足以触发肌动蛋白缓慢和持续地募集到树突棘中。相反，电压门控钙通道的开放迅速且可逆地增强了体细胞外周的皮质肌动蛋白，而不是棘部的皮质肌动蛋白，这与体细胞[Ca~(2+)](I)的高瞬时升高相一致。这些数据表明，由N-甲基-D-天冬氨酸受体和电压门控钙通道激活所触发的[Ca~(2+)](I)的时空动力学为活性依赖性肌动蛋白重构提供了分子基础。
Cytoskeleton is believed to contribute to activity-dependent processes underlying neuronal plasticity, such as regulations of cellular morphology and localization of signaling proteins. However, how neuronal activity controls actin cytoskeleton remains obscure. Taking advantage of confocal imaging of enhanced GFP-actin in the primary culture of hippocampal neurons, we show that synaptic activity induces multiple types of actin reorganization, both at the spines and at the somatic periphery. Activation of N-methyl-D-aspartate receptors, accompanied with a local rise in [Ca2+](i), was sufficient to trigger a slow and sustained recruitment of actin into dendritic spines. In contrast, opening of voltage-gated Ca2+ channels rapidly and reversibly enhanced cortical actin at the somatic periphery but not in the spines, in keeping with a high transient rise in somatic [Ca2+](i). These data suggest that spatiotemporal dynamics of [Ca2+](i), triggered by activation of N-methyl-D-aspartate receptors and voltage-gated Ca2+ channels, provides the molecular basis for activity-de pendent actin remodeling.