Transcriptome analysis of Helicoverpa armigera male hairpencils: Alcohol biosynthesis and requirement for mating success
Transcriptome analysis of Helicoverpa armigera male hairpencils: Alcohol biosynthesis and requirement for mating success
复制标题
雄性毛笔棉铃虫的转录组分析：酒精生物合成和交配成功的要求
DOI：
10.1016/j.ibmb.2017.07.001
复制
发表时间：
2017-08-01
影响因子：
3.8
通讯作者：
An, Shiheng
中科院分区：
文献类型：
--
作者：
Du, Mengfang;Zhao, Wenhui;An, Shiheng
许多雌性动物使用不同的策略来评估雄性的质量，以提高自己的生殖适应性。在飞蛾中，雌性通常使用化学信号（性信息素）来吸引远处的雄性。一旦雄性靠近雌性，它们就会释放出近距离的信息素，以促进雌性的接受。然而，有关雄性性信息素生物合成及其促进雌性接受的作用的详细机制尚未得到充分的描述。本研究通过对棉铃虫雄性发夹的转录组分析，筛选了一系列候选基因。通过药物抑制剂和rnai介导的敲低实验，我们证明Ca2+和环AMP参与信息素生物合成激活神经肽（PBAN）诱导的雄性性信息素生物合成。对雄性性信息素生物合成途径候选酶的功能分析表明，通过rnai介导的敲低乙酰辅酶a羧化酶、δ 11-去饱和酶和脂肪酰基还原酶2的mRNA水平降低，导致脂肪酰基醇的产生和雌性接受效果显著降低。我们的研究结果证明了脂肪酰基醇生物合成途径在pban诱导的雄性性信息素生物合成中的重要作用，以及发胶化合物在雌性交配接受中的重要性。(C) 2017 Elsevier Ltd.版权所有。
Many female animals use different strategies to assess male quality to increase their own reproductive fitness. In moths, females usually use chemical signals (sex pheromones) to attract males from a distance. Once males approach a female, they release close range pheromones from hairpencils to facilitate female acceptance. However, detailed mechanisms involved in male sex pheromone biosynthesis and its action in promoting female acceptance have not yet been fully characterized. This study screened a series of candidate genes via a transcriptome analysis of the male hairpencil of Helicoverpa armigera. Using pharmacological inhibitor and RNAi-mediated knockdown assays, we demonstrated that Ca2+ and cyclic AMP were involved in pheromone biosynthesis activating neuropeptide (PBAN)-induced male sex pheromone biosynthesis. The functional analysis of candidate enzymes involved in the male sex pheromone biosynthesis pathway demonstrated that a decreased mRNA levels of acetyl-CoA carboxylase, Delta 11-desaturase, and fatty-acyl reductase 2 by RNAi-mediated knockdown led to a significant decrease in the production of fatty acyl alcohols and the efficacy of female acceptance. Our results demonstrated the important role of the fatty acyl alcohol biosynthetic pathway in a PBAN-induced male sex pheromone biosynthesis and the importance of hairpencil compounds in female mating acceptance. (C) 2017 Elsevier Ltd. All rights reserved.