Porcine Pluripotency Cell Signaling Develops From the Inner Cell Mass to the Epiblast During Early Development
Porcine Pluripotency Cell Signaling Develops From the Inner Cell Mass to the Epiblast During Early Development
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DOI：
10.1002/dvdy.22027
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发表时间：
2009-08-01
影响因子：
2.5
通讯作者：
Hyttel, Poul
中科院分区：
文献类型：
--
作者：
Hall, Vanessa J.;Christensen, Josef;Hyttel, Poul
调控猪胚胎干细胞和胚胎多能性的信号机制尚不清楚。在这项研究中，我们的特点在体内猪内细胞团和后期上胚层的细胞信号。我们评估了OCT 4、NANOG、SOX 2、JAK/STAT通路内的基因（LIF、LIFR、GP 130）、FGF通路（bFGF、FGFR 1、FGFR 2）、BMP通路（BMP 4）和下游激活基因（STAT 3、c-Myc、c-Fos和SMAD 4）的表达。我们发现在猪胚胎发育过程中存在两种不同的表达谱。D 6猪胚泡（内细胞团阶段）在所分析的大多数基因中缺乏表达，OCT 4除外。相反，D11外胚层表达12个基因中的10个。免疫细胞化学证实LIFR和bFGF不在上胚层中表达，但在滋养外胚层中表达。这些发现揭示了与维持人胚胎干细胞多能性相关的细胞信号传导在猪外胚层中是可检测的，但在内细胞团中不是。发展动态238.2014-2024，2009年。(C)2009 Wiley-Liss，Inc.
The signaling mechanisms regulating pluripotency in porcine embryonic stem cells and embryos are unknown. In this study, we characterize cell signaling in the in-vivo porcine inner cell mass and later-stage epiblast. We evaluate expression of OCT4, NANOG, SOX2, genes within the JAK/STAT pathway (LIF, LIFR, GP130), FGF pathway (bFGF, FGFR1, FGFR2), BMP pathway (BMP4), and downstream-activated genes (STAT3, c-Myc, c-Fos, and SMAD4). We discovered two different expression profiles exist in the developing porcine embryo. The D6 porcine blastocyst (inner cell mass stage) is devoid in the expression of most genes analyzed, with the exception of OCT4. In contrast, the D11 epiblast expressed 10 of the 12 genes investigated. Immunocytochemistry confirmed LIFR and bFGF was not expressed in the epiblast, but within the trophectoderm. These findings reveal cell signaling associated with maintaining pluripotency in human embryonic stem cells is detectable in the porcine epiblast, but not in the inner cell mass. Developmental Dynamics 238.2014-2024, 2009. (C) 2009 Wiley-Liss, Inc.