Human myometrial gene expression before and during parturition
Human myometrial gene expression before and during parturition
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DOI：
10.1095/biolreprod.104.032979
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发表时间：
2005-03-01
影响因子：
3.6
通讯作者：
Word, RA
中科院分区：
文献类型：
--
作者：
Havelock, OC;Keller, P;Word, RA
确定分娩过程中子宫肌层基因表达的时空变化可能会进一步了解分娩过程中子宫肌层收缩的协调调节。本研究的目的是使用功能基因组学方法比较分娩前和分娩后孕妇子宫底肌层的基因表达谱，并进一步表征三个被认为在分娩中重要的基因的时空表达模式。从5名分娩妇女和5名未分娩妇女中分离子宫底肌层mRNA，并使用具有9182个cDNA元件的人UniGEM-V微阵列进行分析。使用实时聚合酶链反应从分娩或未分娩的孕妇子宫肌层RNA被用来检查三个基因的mRNA水平，即，胰高血糖素-内过氧化物合酶2（PTGS 2），钙颗粒蛋白B（S100 A9）和催产素受体（OXTR）。这些基因的空间表达模式在整个妊娠子宫分娩前后也被确定。通过免疫组织化学分析子宫颈和子宫肌层内环氧合酶-2（也称为PTGS 2）和S100 A9的免疫定位。在分娩开始时，子宫底肌层组织中很少有基因差异表达。然而，似乎有一个子集的基因在分娩级联重要。S100 A9的细胞特性、其空间定位以及在分娩妇女子宫颈和子宫肌层中的显著增加表明，该蛋白质可能在人类分娩的启动或传播中非常重要。
Identification of temporal and spatial changes in myometrial gene expression during parturition may further the understanding of the coordinated regulation of myometrial contractions during parturition. The objective of this study was to compare the gene expression profiles of human fundal myometrium from pregnant women before and after the onset of labor using a functional genomics approach, and to further characterize the spatial and temporal expression patterns of three genes believed to be important in parturition. Fundal myometrial mRNA was isolated from five women in labor and five women not in labor, and analyzed using human UniGEM-V microarrays with 9182 cDNA elements. Real-time polymerase chain reaction using myometrial RNA from pregnant women in labor or not in labor was used to examine mRNA levels for three of the genes; namely, prostaglandin-endoperoxide synthase 2 (PTGS2), calgranulin B (S100A9), and oxytocin receptor (OXTR). The spatial expression pattern of these genes throughout the pregnant uterus before and after labor was also determined. Immunolocalization of cyclooxygenase-2 (also known as PTGS2) and S100A9 within the uterine cervix and myometrium were analyzed by immunohistochemistry. Few genes were differentially expressed in fundal myometrial tissues at term with the onset of labor. However, there appears to be a subset of genes important in the parturition cascade. The cellular properties of S100A9, its spatial localization, and dramatic increase in cervix and myometrium of women in labor suggest that this protein may be very important in the initiation or propagation of human labor.