Interactions between the Escherichia coli cyclic AMP receptor protein and RNA polymerase at Class II promoters-猫眼课题宝

大肠杆菌环 AMP 受体蛋白与 II 类启动子处的 RNA 聚合酶之间的相互作用

DOI：

发表时间：

1993

期刊：

Molecular Microbiology

影响因子：

3.6

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

David West;Roy Williams;V. Rhodius;A. Bell;Naveen Sharma;C. Zou;N. Fujita;A. Ishihama;S. Busby

在不同的环AMP受体蛋白（crp）依赖的II类启动子上，已经测量了crp中许多突变的影响，其中crp结合位点集中在转录起点上游的411/2碱基对附近。氨基酸取代HL159和TA158导致CRP依赖性激活减少，但减少程度因II类启动子而异。RNA聚合酶α亚基C端缺失可抑制HL159和TA158的作用。评估了α的C端在这些启动子中的作用。E58、K52和E96的其他变化特别影响II类启动子的CRP活性，并讨论了它们的作用。

The effects of a number of mutations in crp have been measured at different cyclic AMP receptor protein (CRP)‐dependent Class II promoters, where the CRP‐binding site is centred around 411/2 base pairs upstream from the transcription start point. The amino acid substitutions HL159 and TA158 result in reduced CRP‐dependent activation, but the reduction varies from one Class II promoter to another. Deletions in the C‐terminus of the RNA polymerase alpha subunit suppress the effects of HL159 and TA158. The role of the C‐terminus of alpha at these promoters is assessed. Other changes at E58, K52 and E96 affect CRP activity specifically at Class II promoters and their role is discussed.

Identification of the activating region of catabolite gene activator protein (CAP): isolation and characterization of mutants of CAP specifically defective in transcription activation.