Deficient cell proliferation in palatal shelf mesenchyme of CL/Fr mouse embryos
Deficient cell proliferation in palatal shelf mesenchyme of CL/Fr mouse embryos
复制标题
DOI：
10.1177/154405910408301012
复制
发表时间：
2004-10-01
影响因子：
7.6
通讯作者：
Taya, Y
中科院分区：
文献类型：
--
作者：
Sasaki, Y;Tanaka, S;Taya, Y
唇裂基因型如何影响继发性腭形成仍知之甚少。本研究的目的是分析CL/Fr小鼠胚胎细胞增殖的区域模式与唇裂。在E13.5时，从CL/Fr正常胚胎（CL/Fr-N）、CL/Fr双侧唇裂胚胎（CL/Fr-BCL）和对照品系的C57 BL胚胎（C57 BL）中解剖成对的腭架。器官培养48小时后，对外植体进行组织学检查。在E13.5通过体内溴脱氧尿苷方法检查腭架中增殖的细胞动力学。CL/Fr-BCL腭融合以及CL/Fr-N腭在体外融合。各组间BrdU阳性细胞的绝对数量和阳性/（阳性+阴性）细胞的比值在腭间质（C57 BL> CL/Fr-N> CL/Fr-BCL）和上皮（C57 BL> CL/Fr-N = CL/Fr-BCL）中存在差异。这些发现表明，腭裂以下减少的细胞增殖的次级腭间充质在CL/Fr小鼠。
How secondary palate formation is affected in the cleft lip genotype remains poorly understood. The purpose of this study was to analyze regional patterns of cell proliferation in CL/Fr mouse embryos with or without cleft lip. Pairs of palatal shelves were dissected at E13.5 from CL/Fr normal embryos (CL/Fr-N), CL/Fr embryos with bilateral cleft lip (CL/Fr-BCL), and a control strain of C57BL embryos (C57BL). The explants were examined histologically after 48 hrs of organ culture. Cell kinetics for proliferation in the palatal shelves was examined at E13.5 by the bromodeoxyuridine method in vivo. The CL/Fr-BCL palates fused as well as the CL/Fr-N palates in vitro. There were inter-group differences in the absolute number of BrdU-positive cells and the ratio of positive/(positive+negative) cells in the palate's mesenchyme ( C57BL > CL/ Fr- N > CL/Fr-BCL) and epithelium ( C57BL > CL/ Fr- N = CL/Fr-BCL). These findings indicate that a cleft palate follows reduced cell proliferation of secondary palatal mesenchyme in CL/Fr mice.