Assessment of Neutrophil Apoptosis
Assessment of Neutrophil Apoptosis
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DOI：
10.1007/978-1-62703-845-4_10
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Lucas, Christopher D.
中科院分区：
文献类型：
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作者：
Dorward, David A.;Rossi, Adriano G.;Lucas, Christopher D.
及时的中性粒细胞凋亡和周围吞噬细胞的细胞清除是急性炎症消退期的重要组成部分。通过细胞凋亡进行的程序性细胞死亡在维持完整细胞膜的情况下发生，以防止在坏死期间发生的组织毒性细胞内产物如蛋白酶和反应性氧化剂物质释放到细胞外环境中。巨噬细胞吞噬导致Toll样受体驱动的促炎介质产生的衰减，进一步促进炎症消退。这一系列事件的失败可能导致组织损伤、慢性炎症和疾病。通过研究体外人中性粒细胞凋亡和吞噬细胞清除，有可能描绘这些过程的调节中的关键控制机制，因此也确定了潜在的治疗靶点。文献中使用多种实验室技术对细胞凋亡信号通路进行了详细描述。在本文中，我们概述了关键的体外试验用于评估中性粒细胞凋亡，激活的关键组成部分的凋亡机制，这些细胞的吞噬清除。
Timely neutrophil apoptosis and cell clearance by surrounding phagocytes are essential components of the resolution phase of acute inflammation. Programmed cell death by apoptosis occurs with maintenance of an intact cell membrane in order to prevent the release of histotoxic intracellular products such as proteases and reactive oxidant species into the extracellular surroundings as occurs during necrosis. Macrophage phagocytosis results in attenuation of Toll-like receptor-driven proinflammatory mediator production further promoting inflammation resolution. Failures in this cascade of events can result in tissue damage, chronic inflammation and disease. By studying human neutrophil apoptosis and phagocytic clearance in vitro, it is possible to delineate key control mechanisms in the regulation of these processes and therefore also identify potential therapeutic targets. Apoptotic signalling pathways are well described in the literature using a variety of laboratory techniques. In this paper, we outline the key in vitro assays used to assess neutrophil apoptosis, activation of key components of the apoptotic machinery, and phagocytic clearance of these cells.