QUANTITATIVE ANALYSIS OF CELL PROLIFERATION AND DIFFERENTIATION IN CORTEX OF POSTNATAL MOUSE CEREBELLUM
QUANTITATIVE ANALYSIS OF CELL PROLIFERATION AND DIFFERENTIATION IN CORTEX OF POSTNATAL MOUSE CEREBELLUM
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DOI：
10.1083/jcb.32.2.277
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发表时间：
1967-01-01
影响因子：
7.8
通讯作者：
FUJITA, S
中科院分区：
文献类型：
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作者：
FUJITA, S
用放射自显影技术研究了10~11日龄小鼠小脑皮质外颗粒层中的生殖细胞(外基质细胞)在重复注射H^-胸腺碱后的发生周期。世代时间19小时，合成前时间8.5小时，DNA合成时间8小时，合成后时间2小时，有丝分裂时间0.5小时。这些增殖细胞占据外颗粒层的外半部，构成外基质层。神经母细胞是从外基质细胞分化而来的，从外基质细胞层迁移出来，聚集在外颗粒层的内侧，形成外外套层。神经母细胞在外膜层的传递时间为28小时。此后，它们进一步迁移到分子层和内腺层。通过长期累积标记的方法，定量地研究了外基质细胞每天产生神经母细胞的速率。结果表明，内颗粒神经元在出生后1~18天全部出现在外颗粒层，其中95%的细胞产生于出生后4~15天。最后，用H^-胸腺嘧啶核苷标记15~16天的外颗粒层细胞及其随后的迁移和发育变化来研究外颗粒层细胞在终末期的命运。比较迁移前后的放射自显影发现，外基质细胞产生了少量的神经胶质细胞。这一发现揭示了它们的多潜能本质。
The generation cycle of germinative cells (external matrix cells) in the external granular layer of the cerebellar cortex of the 10- to 11-day-old mouse was studied by radio-autography following repeated injections of H^-thymldine. The generation time is 19 hr, presynthetic time 8.5 hr, DNA-synthetic time 8 hr, postsynthetic time 2 hr, and mitotic time 0.5 hr. These proliferating cells occupy the outer half of the external granular layer and make up the external matrix layer. Neuroblasts are differentiated from the external matrix cell, migrate out from the layer and accumulate in the inner half of the external granular layer to form the external mantle layer. The transit time of the neuroblasts in the external mantle layer is 28 hr. Thereafter, they migrate farther into the molecular layer and the internal glandular layer. By means of long-term cumulative labeling, the rate of daily production of neuroblasts from the external matrix cell is studied in quantitative terms. It becomes clear that the entire population of the inner granule neurons arises postnatally in the external granular layer between 1 and 18 days of age and that 95% of them is produced between postnatal days 4 and 15. Finally, the fate of the cells in the external granular layer at Us terminal stage was studied by marking the cells with H^-thymidine during 15-16 days of life and following their subsequent migration and developmental changes up to 21 days of life. Comparison of radioautographs taken before and after the migration disclosed that the external matrix cells give rise to a small number of neuroglia cells. This finding revealed their multipotential nature.